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BACKGROUND

The preservation of cut flowers, particularly Gerbera jamesonii, is crucial for maintaining their aesthetic value and extending vase life in the floriculture industry. To address this challenge, this study investigated the effects of melatonin (Mel) and encapsulated melatonin with nanochitosan (nCS-Mel) as preservative solutions on cut Gerbera jamesonii cv. 'Terra kalina' flowers. In research, we examined various physiological and biochemical parameters, including relative water content, membrane stability index, carbohydrate content, and antioxidant enzyme activities, to evaluate the efficacy of these treatments in prolonging the vase life and quality of cut gerbera flowers under controlled environmental conditions.

RESULTS

Our results demonstrated that cut Gerbera jamesonii flowers maintained in vase solutions containing 0.1 and 0.5 mM nCS-Mel exhibited enhanced preservation of cell membrane integrity and anthocyanin content, while also maintaining higher levels of carbohydrates and total flavonoids in petals at the conclusion of their vase life. A decline in petal relative water content and protein levels was observed concomitantly with petal senescence, whereas total phenolic compounds showed an increase. The hydrogen peroxide (HO) content in petals exhibited an upward trend during vase life in control specimens, but this effect was mitigated in treatments containing melatonin. Although malondialdehyde (MDA) content generally increased throughout the vase life period, flowers subjected to either Mel or nCS-Mel treatments displayed reduced MDA accumulation. The activity of catalase (CAT) demonstrated an increasing trend during vase life, with the maximum activity observed in Gerbera flowers treated with 0.1 mM nCS-Mel. A similar upward trend was noted for superoxide dismutase (SOD) activity, with flowers in 0.5 mM nCS-Mel treatment exhibiting peak SOD values on day 12 relative to control and other treatments. Peroxidase (POD) activity also increased across all treatments, with particularly pronounced effects in vase solutions containing 0.1 mM Mel and nCS-Mel. Notably, flowers placed in vase solutions containing 0.1 mM nCS-Mel, followed by 0.5 mM nCS-Mel and 0.1 mM Mel, exhibited the most prolonged vase life, extending up to 12, 10.66, and 10.33 days, respectively, under room temperature conditions.

CONCLUSIONS

The application of nanoencapsulated melatonin as a vase solution for cut Gerbera jamesonii flowers demonstrates significant potential in extending vase life and maintaining flower quality through enhanced preservation of cellular integrity, antioxidant activity, and biochemical parameters. This innovative approach not only outperforms conventional treatments but also presents a more environmentally friendly alternative to traditional antimicrobial preservatives and sugars, offering a promising solution for the floriculture industry to improve cut flower longevity and reduce ecological impact.

BACKGROUND

RESULTS

CONCLUSIONS

背景

对于花卉产业来说，保持切花，特别是非洲菊的美观和延长瓶插寿命至关重要。为了解决这个问题，本研究调查了褪黑素（Mel）和包被纳米壳聚糖的褪黑素（nCS-Mel）作为保鲜剂对切花非洲菊‘Terra kalina’的影响。在研究中，我们检查了各种生理和生化参数，包括相对水含量、膜稳定性指数、碳水化合物含量和抗氧化酶活性，以评估这些处理在控制环境条件下延长切花非洲菊瓶插寿命和品质的效果。

结果

我们的结果表明，在含有 0.1 和 0.5 mM nCS-Mel 的花瓶溶液中保存的切花非洲菊保持了细胞膜完整性和花色苷含量的增强保存，同时在花瓣寿命结束时也保持了较高水平的碳水化合物和总类黄酮。花瓣相对水含量和蛋白质水平的下降与花瓣衰老同时发生，而总酚类化合物则呈上升趋势。在对照标本的瓶插过程中，花瓣中的过氧化氢（HO）含量呈上升趋势，但在含有褪黑素的处理中，这种影响得到了缓解。虽然丙二醛（MDA）含量在整个瓶插寿命期间通常会增加，但用 Mel 或 nCS-Mel 处理的花朵显示 MDA 积累减少。在瓶插过程中，过氧化氢酶（CAT）的活性呈上升趋势，在 0.1 mM nCS-Mel 处理的非洲菊中观察到最大的 CAT 活性。超氧化物歧化酶（SOD）活性也呈上升趋势，在 0.5 mM nCS-Mel 处理的花朵中，与对照和其他处理相比，第 12 天达到 SOD 值峰值。过氧化物酶（POD）活性在所有处理中均增加，在含有 0.1 mM Mel 和 nCS-Mel 的花瓶溶液中尤为明显。值得注意的是，置于含有 0.1 mM nCS-Mel 的花瓶溶液中的花朵，随后置于 0.5 mM nCS-Mel 和 0.1 mM Mel 的花瓶溶液中，在室温条件下分别可延长至 12、10.66 和 10.33 天，表现出最长的瓶插寿命。