The First Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, China.
School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.
BMC Cancer. 2024 Oct 29;24(1):1331. doi: 10.1186/s12885-024-13077-w.
This study employs integrated bioinformatics analysis and in vitro cellular experiments to elucidate the role of Synaptotagmin-12 (SYT12) in the progression of gastric cancer.
We utilized databases and platforms such as Xiantao Academic Tools, UALCAN, Kaplan-Meier plotter analysis, and The Cancer Genome Atlas (TCGA) to extract datasets on SYT12 in gastric cancer. We analyzed the relationship between SYT12 expression and the clinicopathological features, prognosis, diagnosis, and immune infiltration of stomach adenocarcinoma (STAD) patients. Verification was conducted using samples from 31 gastric cancer patients. Additionally, in vitro cellular experiments were performed to determine the role and potential mechanisms of SYT12 in the malignant behavior of gastric cancer cells.
Comprehensive bioinformatics analysis indicated that SYT12 is highly expressed in most cancers and is associated with promoter DeoxyriboNucleic Acid (DNA) methylation levels. SYT12 expression correlated with clinicopathological features, immune cell infiltration, immune checkpoint gene expression, and poor prognosis in STAD patients. In vitro experiments suggest that SYT12 may promote the proliferation and migration of gastric cancer cells by inducing epithelial-mesenchymal transition (EMT).
This study highlights the significant role of SYT12 in gastric cancer, suggesting its potential as a new target for early diagnosis, treatment, immunological, and prognostic evaluation in gastric cancer, offering new insights for precision medicine in this disease.
本研究采用综合生物信息学分析和体外细胞实验，阐明突触结合蛋白 12（SYT12）在胃癌进展中的作用。
我们利用 Xiantao Academic Tools、UALCAN、Kaplan-Meier plotter 分析和癌症基因组图谱（TCGA）等数据库和平台，提取胃癌中 SYT12 的数据集。我们分析了 SYT12 表达与胃腺癌（STAD）患者临床病理特征、预后、诊断和免疫浸润的关系。使用 31 例胃癌患者的样本进行了验证。此外，还进行了体外细胞实验，以确定 SYT12 在胃癌细胞恶性行为中的作用和潜在机制。
综合生物信息学分析表明，SYT12 在大多数癌症中高表达，并与启动子脱氧核糖核酸（DNA）甲基化水平相关。SYT12 的表达与 STAD 患者的临床病理特征、免疫细胞浸润、免疫检查点基因表达和不良预后相关。体外实验表明，SYT12 可能通过诱导上皮-间充质转化（EMT）促进胃癌细胞的增殖和迁移。
本研究强调了 SYT12 在胃癌中的重要作用，提示其可能成为胃癌早期诊断、治疗、免疫和预后评估的新靶点，为该疾病的精准医学提供了新的见解。
