微粒体结合细胞色素P-450 LM2的磷酸化作用

Phosphorylation of microsome-bound cytochrome P-450 LM2.

作者信息

Pyerin W, Marx M, Taniguchi H

出版信息

Biochem Biophys Res Commun. 1986 Jan 29;134(2):461-8. doi: 10.1016/s0006-291x(86)80443-0.

Abstract

The phosphorylation of a microsomal protein of rabbit liver by catalytic subunit of cyclic AMP-dependent protein kinase was shown, and the protein was identified as cytochrome P-450 LM2 on basis of comparative peptide-mapping. Acid hydrolysis of microsome-bound phosphorylated cytochrome P-450 revealed that phosphorylation occurred exclusively on serine residues. This serine residue was identified as the same residue phosphorylated in purified, soluble P-450, that is, serine in position 128.

摘要

已证实环磷酸腺苷依赖性蛋白激酶的催化亚基可使兔肝微粒体蛋白发生磷酸化，并且基于比较肽图分析将该蛋白鉴定为细胞色素P - 450 LM2。对微粒体结合的磷酸化细胞色素P - 450进行酸水解后发现，磷酸化仅发生在丝氨酸残基上。该丝氨酸残基被鉴定为与纯化的可溶性P - 450中被磷酸化的残基相同，即第128位的丝氨酸。