Raman Deevya, Wernet Nicole, Gang Spencer, Troemel Emily
Cell and Developmental Biology, University of California, San Diego, San Diego, CA, United States.
Molecular Biology Department, Colorado College, Colorado Springs, Colorado, United States.
MicroPubl Biol. 2024 Oct 15;2024. doi: 10.17912/micropub.biology.001325. eCollection 2024.
Microsporidia are common natural pathogens of the nematode . Infection of by the microsporidian species leads to induction of the Intracellular Pathogen Response (IPR), including transcriptional upregulation of 26 genes. The divergent ' ' sequence signature is conserved with humans, but PALS proteins have unknown biochemical functions. So far, none of the 26 induced genes have a demonstrated role in immunity. Here, we use RNAseq data, RNA interference, and CRISPR/Cas9 mutant analysis to identify the -induced gene as an immune gene that provides defense against microsporidia infection in .
微孢子虫是线虫常见的天然病原体。线虫被微孢子虫物种感染会导致细胞内病原体反应（IPR）的诱导，包括26个基因的转录上调。不同的“ ”序列特征在人类中是保守的，但PALS蛋白的生化功能尚不清楚。到目前为止，26个诱导的基因中没有一个在免疫中发挥已证实的作用。在这里，我们使用RNA测序数据、RNA干扰和CRISPR/Cas9突变分析来鉴定被诱导的基因是一种免疫基因，它能对线虫中的微孢子虫感染提供防御。
