转录组分析揭示双酚 AP 暴露诱导斑马鱼胚胎的炎症反应
Bisphenol AP Exposure-Induced Inflammatory Response in Zebrafish Embryos Revealed by Transcriptomic Analysis.
作者信息
Ding Qingjiang, Wang Hailin
机构信息
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, China.
出版信息
Environ Health (Wash). 2024 May 1;2(6):373-380. doi: 10.1021/envhealth.4c00028. eCollection 2024 Jun 21.
Bisphenol AP (BPAP) is a bisphenol analogue that is used as a substitute for BPA in industrial production and is now commonly found in the environment. Currently, there is limited information available on its developmental toxicity in aquatic organisms. This study exploited zebrafish embryos to investigate the adverse effects of BPAP exposure during their early life stages. The results revealed that BPAP had a median lethal concentration (LC) of 36, 22, and 8.5 μmol/L for zebrafish embryos at 24, 48, and 72 h post fertilization (hpf), respectively. Additionally, exposure to BPAP led to abnormal development in zebrafish embryos, including hatching delay, malformation, and inhibition of spontaneous movements. Transcriptomic analysis further revealed that BPAP exposure upregulated the mRNA expression levels of genes related to inflammatory pathways, such as , , and , indicating the induction of an inflammatory response. Overall, our findings provide early and valuable insights into the environmental and health risks associated with BPAP exposure.
双酚 AP（BPAP）是一种双酚类似物，在工业生产中用作双酚 A（BPA）的替代品，现在在环境中普遍存在。目前，关于其对水生生物发育毒性的信息有限。本研究利用斑马鱼胚胎来研究在其生命早期阶段暴露于 BPAP 的不良影响。结果显示，对于受精后 24、48 和 72 小时（hpf）的斑马鱼胚胎，BPAP 的半数致死浓度（LC）分别为 36、22 和 8.5 μmol/L。此外，暴露于 BPAP 导致斑马鱼胚胎发育异常，包括孵化延迟、畸形和自发运动受到抑制。转录组分析进一步表明，暴露于 BPAP 会上调与炎症途径相关基因的 mRNA 表达水平，如 、 和 ，表明诱导了炎症反应。总体而言，我们的研究结果为与 BPAP 暴露相关的环境和健康风险提供了早期且有价值的见解。
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