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紫檀芪通过诱导凋亡抑制头颈癌细胞增殖。

Pterostilbene suppresses head and neck cancer cell proliferation via induction of apoptosis.

作者信息

Özdaş Talih, Özdaş Sibel, Canatar İpek, Kaypak Erdem

机构信息

Department of ENT, Adana City Training and Research Hospital, Health Science University, Adana, Turkiye.

Department of Bioengineering, Faculty of Engineering Sciences, Adana Alparslan Türkeş Science and Technology University, Adana, Turkiye.

出版信息

Turk J Biol. 2024 Aug 27;48(5):319-337. doi: 10.55730/1300-0152.2708. eCollection 2024.

DOI:10.55730/1300-0152.2708
PMID:39474042
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11518375/
Abstract

BACKGROUND/AIM: Head and neck cancer (HNC) is one of the most prevalent causes of death worldwide, and so discovering anticancer agents for its treatment is very important. Pterostilbene (PS) is a trans-stilbene reported to be beneficial in managing various cancers. The objective of the study was to evaluate the cytotoxic, antiproliferative, proapoptotic, and antimigrative effect of PS on HEp-2, SCC-90, SCC-9, FaDu, and Detroit-551 cell lines.

MATERIALS AND METHODS

MTT and live/dead assays were employed to assess cell viability, while a cell migration test was performed to evaluate wound healing capacity. The mRNA, protein, and intracellular expression levels of , , and genes were evaluated by real-time PCR, western blotting, and immunofluorescence staining. Annexin V-PI staining was conducted to assess the amounts of viable, apoptotic, and necrotic cells.

RESULTS

The results revealed that PS displayed cytotoxic, antiproliferative activity in a dose-dependent manner in HNC cells by upregulating and while downregulating in the apoptotic pathway. The proapoptotic properties were confirmed by the annexin-V-IP results. Moreover, PS displayed a significant suppressive efficacy on the migration capacity of HNC cells.

CONCLUSION

The present study provides proof that PS has the prospective to be improved as an attractive anticancer agent against HNC following advanced studies.

摘要

背景/目的：头颈癌（HNC）是全球最常见的死亡原因之一，因此发现用于治疗的抗癌药物非常重要。紫檀芪（PS）是一种反式芪，据报道对多种癌症的治疗有益。本研究的目的是评估PS对HEp-2、SCC-90、SCC-9、FaDu和Detroit-551细胞系的细胞毒性、抗增殖、促凋亡和抗迁移作用。

材料与方法

采用MTT和活/死检测法评估细胞活力，同时进行细胞迁移试验以评估伤口愈合能力。通过实时PCR、蛋白质印迹和免疫荧光染色评估 、 和 基因的mRNA、蛋白质和细胞内表达水平。进行膜联蛋白V-碘化丙啶染色以评估活细胞、凋亡细胞和坏死细胞的数量。

结果

结果显示，PS通过上调凋亡途径中的 和 并下调 ，以剂量依赖的方式在HNC细胞中表现出细胞毒性和抗增殖活性。膜联蛋白-V-IP结果证实了其促凋亡特性。此外，PS对HNC细胞的迁移能力显示出显著的抑制作用。

结论

本研究证明，经过进一步研究，PS有望成为一种有吸引力的抗HNC抗癌药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/2924dc35ffb6/tjb-48-05-319f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/377213616122/tjb-48-05-319f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/2924dc35ffb6/tjb-48-05-319f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/377213616122/tjb-48-05-319f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/c57ab056fb51/tjb-48-05-319f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/ae56493e03af/tjb-48-05-319f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/88db39b7b67a/tjb-48-05-319f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/b4ade590b362/tjb-48-05-319f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/1458903efe2f/tjb-48-05-319f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/c4dd793c6e01/tjb-48-05-319f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/7ad080db050a/tjb-48-05-319f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/40c5185ce90c/tjb-48-05-319f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/5c8cf151464d/tjb-48-05-319f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/1db3ebed8409/tjb-48-05-319f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/7107f29669f9/tjb-48-05-319f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b01/11518375/2924dc35ffb6/tjb-48-05-319f13.jpg

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