Kalhor Naser, Fazaeli Hoda, Asl Faezeh Davoodi, Sheikholeslami Azar, Hoseini Seyed Jalal Eshagh, Sheykhhasan Mohsen
Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Qom, Iran.
Department of Surgery, Qom University of Medical Science, Qom, Iran.
Int J Cell Biol. 2024 Oct 21;2024:1022338. doi: 10.1155/2024/1022338. eCollection 2024.
Recent research indicates that mesenchymal stem cells (MSCs), known for their anti-inflammatory and anti-infectious properties, could be a promising alternative for treating inflammatory diseases such as inflammatory bowel disease (IBD). This study examined how MSCs and their derivatives, when cocultured with interleukin-1 (IL-1)-stimulated Caco-2 cells, affect the expression of genes related to inflammation, microbes, and apoptosis. In the experiment, Caco-2 cells were exposed to 10 ng/mL of IL-1 for 24 h. MSCs were sourced from human bone marrow, adipose tissue (AD-MSC), and menstrual blood. These MSCs and their conditioned medium (CM) were then cocultured with the IL-1-induced Caco-2 cells. After 48 h, gene expression levels were analyzed using real-time PCR, and the data were statistically evaluated using -tests, -Mann-Whitney, and Tukey's post hoc analyses. The results indicated that IL-1 at 10 ng/mL was the optimal concentration for inducing Caco-2 cells with the highest viability and minimal damage. Among the MSCs tested, AD-MSCs were the most effective in regulating gene expression. Specifically, AD-MSC treatment significantly reduced the mRNA expression of TNF- and IL-1, both of which are crucial in sustaining inflammatory responses ( ≤ 0.05). This study concludes that AD-MSCs have superior effects compared to other MSC sources in modulating genes associated with inflammation, antibacterial effects, and apoptosis in an in vitro model of IBD using Caco-2 cells.
近期研究表明，间充质干细胞（MSCs）以其抗炎和抗感染特性而闻名，可能是治疗炎症性疾病如炎症性肠病（IBD）的一种有前景的替代方法。本研究考察了MSCs及其衍生物与白细胞介素-1（IL-1）刺激的Caco-2细胞共培养时，如何影响与炎症、微生物和细胞凋亡相关基因的表达。在实验中，Caco-2细胞暴露于10 ng/mL的IL-1中24小时。MSCs来源于人骨髓、脂肪组织（AD-MSC）和月经血。然后将这些MSCs及其条件培养基（CM）与IL-1诱导的Caco-2细胞共培养。48小时后，使用实时PCR分析基因表达水平，并使用t检验、曼-惠特尼检验和Tukey事后分析对数据进行统计学评估。结果表明，10 ng/mL的IL-1是诱导Caco-2细胞活力最高且损伤最小的最佳浓度。在所测试的MSCs中，AD-MSCs在调节基因表达方面最有效。具体而言，AD-MSC处理显著降低了TNF-α和IL-1的mRNA表达，这两者在维持炎症反应中都至关重要（P≤0.05）。本研究得出结论，在使用Caco-2细胞的IBD体外模型中，与其他MSC来源相比，AD-MSCs在调节与炎症、抗菌作用和细胞凋亡相关的基因方面具有更优越的效果。
