Prevalence of in a Public Building Water Plumbing System During COVID-19 Lockdown.

Although water stagnation is widely accepted as an essential factor supporting growth in plumbing systems and "water flashing" has become a common action for water quality control, additional monitoring data in practical spaces are still needed to back up this recommendation. The lockdown of public buildings during the COVID-19 pandemic provided an ideal time window to collect such data on a large scale. This study investigated how the long-term lockdown of a public building and the subsequent water stagnation impact water quality and the population of in water. From June 2020 to May 2021, 192 water samples were collected from a public building during the lockdown and reopening due to the COVID-19 pandemic. Each water sample was assessed for common physicochemical characteristics. Concentrations of and three species of free-living amoeba (FLA) ( spp., , and ) were monitored by qPCR. The data suggest that long-term stagnation promotes the population of spp., spp., and . Notable associations were observed between and FLA. These relationships were impacted by stagnation. These results provide important evidence that can inform future water quality management actions to minimize the risk of outbreaks by avoiding the occurrence of water stagnation.