Brown University, School of Engineering, Providence, Rhode Island, United States.
Brown University, Department of Pathology and Laboratory Medicine, Providence, Rhode Island, United States.
J Biomed Opt. 2024 Oct;29(10):106002. doi: 10.1117/1.JBO.29.10.106002. Epub 2024 Oct 29.
The polarimetric properties of biological tissues are often difficult to ascertain independent of their complex structural and organizational features. Conventional polarimetric tissue phantoms have well-characterized optical properties but are overly simplified. We demonstrate that an innovative, biologically sourced, engineered tissue construct better recapitulates the desired structural and polarimetric properties of native collagenous tissues, with the added benefit of potential tunability of the polarimetric response. We bridge the gap between non-biological polarimetric phantoms and native tissues.
We aim to evaluate a synthesized tissue construct for its effectiveness as a phantom that mimics the polarimetric properties in typical collagenous tissues.
We use a fibroblast-derived, ring-shaped engineered tissue construct as an innovative tissue phantom for polarimetric imaging. We perform polarimetry measurements and subsequent analysis using the Mueller matrix decomposition and Mueller matrix transformation methods. Scalar polarimetric parameters of the engineered tissue are analyzed at different time points for both a control group and for those treated with the transforming growth factor . Second-harmonic generation (SHG) imaging and three-dimensional collagen fiber organization analysis are also applied.
We identify linear retardance and circular depolarization as the parameters that are most sensitive to the tissue culture time and the addition of . Aside from a statistically significant increase over time, the behavior of linear retardance and circular depolarization indicates that the addition of accelerates the growth of the engineered tissue, which is consistent with expectations. We also find through SHG images that collagen fiber organization becomes more aligned over time but is not susceptible to the addition of .
The engineered tissue construct exhibits changes in polarimetric properties, especially linear retardance and circular depolarization, over culture time and under treatments. This tissue construct has the potential to act as a controlled modular optical phantom for polarimetric-based methods.
生物组织的偏振特性通常难以确定，而其复杂的结构和组织特征则难以确定。传统的偏振组织体模具有良好的光学特性，但过于简化。我们证明了一种创新的、源自生物的工程组织构建体更好地再现了天然胶原组织所需的结构和偏振特性，并且具有偏振响应的潜在可调性。我们在非生物偏振体模和天然组织之间架起了桥梁。
我们旨在评估一种合成组织构建体作为模拟典型胶原组织偏振特性的幻影的有效性。
我们使用源自成纤维细胞的环形工程组织构建体作为偏振成像的创新组织幻影。我们使用 Mueller 矩阵分解和 Mueller 矩阵变换方法进行偏振测量和后续分析。在不同的时间点分析工程组织的标量偏振参数，包括对照组和用转化生长因子 处理的组。还应用了二次谐波产生（SHG）成像和三维胶原纤维组织分析。
我们确定线性延迟和圆偏极化是对组织培养时间和添加 最敏感的参数。除了随时间的统计学显著增加外，线性延迟和圆偏极化的行为表明添加 加速了工程组织的生长，这与预期一致。我们还通过 SHG 图像发现，胶原纤维组织随时间变得更加一致，但不受添加 的影响。
工程组织构建体在培养时间和 处理下表现出偏振特性的变化，特别是线性延迟和圆偏极化。这种组织构建体有可能作为基于偏振的方法的可控模块化光学幻影。
