缺血修饰白蛋白在急性胰腺炎预后中的作用及其与 NF-κB 介导的炎症反应的相关性。

The role of ischaemia-modified albumin in the prognosis of acute pancreatitis and its correlation with the NF-B-mediated inflammatory response.

机构信息

Department of Hepatobiliary and Pancreatic Surgery, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Province, China.

出版信息

J Int Med Res. 2024 Oct;52(10):3000605241287163. doi: 10.1177/03000605241287163.

OBJECTIVE

To investigate the correlation between the serum levels of ischaemia-modified albumin (IMA) and disease severity in rats with acute pancreatitis (AP).

METHODS

A rat AP model was established and blood samples from each group were analysed at different time points. After the experiment, the pancreatic tissues of the rats were collected for pathological examination and the measurement of protein levels of NF-κB and NF-κB p65. Serum levels of amylase (α-AMY), tumour necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-8 were also compared between groups of rats.

RESULTS

The serum IMA concentration in the severe acute pancreatitis (SAP) group was greater than that in the mild acute pancreatitis (MAP) group. The levels of the NF-κB and NF-κB p65 proteins were increased in the MAP and SAP groups in a time-dependent manner. α-AMY, TNF-α and IL-6 were increased at all time points in the MAP and SAP groups. The increases were greatest at 24 h in the SAP group. In terms of pathological changes in the pancreas, renal and lung tissues, the damage in the SAP group was more obvious than that in the MAP group.

CONCLUSIONS

Serum IMA level was associated with inflammatory markers and NF-κB p65 in rats with AP.

目的

探讨急性胰腺炎（AP）大鼠血清缺血修饰白蛋白（IMA）水平与疾病严重程度的相关性。

方法

建立大鼠 AP 模型，在不同时间点分析各组血液样本。实验结束后，收集大鼠胰腺组织进行病理检查，并测定 NF-κB 和 NF-κB p65 蛋白水平。比较各组大鼠血清淀粉酶（α-AMY）、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6 和 IL-8 水平。

结果

重症急性胰腺炎（SAP）组血清 IMA 浓度大于轻症急性胰腺炎（MAP）组。MAP 和 SAP 组 NF-κB 和 NF-κB p65 蛋白水平随时间呈依赖性增加。MAP 和 SAP 组的 α-AMY、TNF-α 和 IL-6 均在各时间点升高，SAP 组在 24 h 时升高最明显。就胰腺、肾和肺组织的病理变化而言，SAP 组的损伤比 MAP 组更明显。