Department of Veterinary, Amasya University Suluova Vocational School, Amasya, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey.
Vet Med Sci. 2024 Nov;10(6):e70101. doi: 10.1002/vms3.70101.
Sperm cryopreservation is a rather complex process that needs to be adapted to wild and domestic bird species to ensure adequate efficiency. This study aimed to determine the usability of different cryoprotectants in the cryopreservation of Gloster canary sperm. For this purpose, sperm samples were collected from 12 2-year-old male Gloster canaries three times a week using cloacal massage for 4 weeks. After individual evaluation, sperm samples from the canaries were combined. Mixed sperm were divided into two groups in the study. Overall, 8% dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were used as cryoprotectants. Sperm samples were drawn into straws after adding Dulbecco's Modified Eagle Medium (DMEM) extender with high glucose ratio and two different cryoprotectants in a 1:1 ratio and frozen to -80°C with liquid nitrogen vapour and then stored in liquid nitrogen at -196°C. Frozen-thawed semen samples were evaluated regarding motility, vitality, plasma membrane integrity (hypoosmotic swelling test [HOST]), density and abnormal spermatozoa rate. The highest motility value after freezing and thawing was determined in the EG group with 31.667% ± 4.773%. In addition, vitality, plasma membrane integrity and normal sperm morphology were statistically significantly higher in the EG-frozen group, whereas head and tail abnormality was low (p < 0.05). This study determined that a DMEM extender containing 8% EG was more advantageous than a DMEM containing DMSO regarding spermatological parameters and could be used for long-term storage of canary sperm.
精子冷冻保存是一个相当复杂的过程，需要适应野生和家养鸟类物种，以确保足够的效率。本研究旨在确定不同冷冻保护剂在格洛斯特金丝雀精子冷冻保存中的可用性。为此，使用直肠按摩每周从 12 只 2 岁雄性格洛斯特金丝雀中收集精子样本 3 次，持续 4 周。经过个体评估后，将金丝雀的精子样本混合在一起。在研究中，混合精子分为两组。总体而言，8%二甲基亚砜（DMSO）和乙二醇（EG）用作冷冻保护剂。在添加高葡萄糖比的 Dulbecco 改良 Eagle 培养基（DMEM） extender 和两种不同的冷冻保护剂 1:1 比例后，将精子样本吸入 straws 中，然后在 -80°C 下用液氮蒸气冷冻，并储存在 -196°C 的液氮中。对冷冻解冻后的精液样本的活力、活力、质膜完整性（低渗肿胀试验[HOST]）、密度和异常精子率进行评估。冷冻和解冻后活力值最高的是 EG 组，为 31.667%±4.773%。此外，EG 冷冻组的活力、质膜完整性和正常精子形态学均显著更高，而头部和尾部异常较低（p<0.05）。本研究确定，含有 8%EG 的 DMEM 添加剂在精子参数方面比含有 DMSO 的 DMEM 更有利，可用于长期储存金丝雀精子。
