Center of Reproductive Medicine, the General Hospital of Southern Theater Command, Guangzhou, 510010China.
Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, 410008China.
Zygote. 2024 Oct;32(5):360-365. doi: 10.1017/S0967199424000340. Epub 2024 Oct 30.
Hydrogen sulfide (HS) has been shown to play a significant role in oxidative stress across various tissues and cells; however, its role in sperm function remains poorly understood. This study aimed to investigate the protective effect of GYY4137, a slow-releasing HS compound, on sperm damage induced by HO. We assessed the effects of GYY4137 on motility, viability, lipid peroxidation and caspase-3 activity in human spermatozoa in vitro following oxidative damage mediated by HO. Spermatozoa from 25 healthy men were selected using a density gradient centrifugation method and cultured in the presence or absence of 10 μM HO, followed by incubation with varying concentrations of GYY4137 (0.625-2.5 μM). After 24 h of incubation, sperm motility, viability, lipid peroxidation, and caspase-3 activity were evaluated. The results indicated that HO adversely affected sperm parameters, reducing motility and viability, while increasing oxidative stress, as evidenced by elevated lipid peroxidation and caspase-3 activity. GYY4137 provided dose-dependent protection against HO-induced oxidative stress (OS). We concluded that supplementation with GYY4137 may offer antioxidant protection during in vitro sperm preparation for assisted reproductive technology.
硫化氢 (HS) 在各种组织和细胞的氧化应激中起着重要作用；然而，其在精子功能中的作用仍知之甚少。本研究旨在探讨一种缓慢释放的 HS 化合物 GYY4137 对 HO 诱导的精子损伤的保护作用。我们评估了 GYY4137 对 HO 介导的氧化损伤后体外人精子活力、活力、脂质过氧化和 caspase-3 活性的影响。采用密度梯度离心法从 25 名健康男性中选择精子，并在存在或不存在 10 μM HO 的情况下培养，然后用不同浓度的 GYY4137（0.625-2.5 μM）孵育。孵育 24 小时后，评估精子活力、活力、脂质过氧化和 caspase-3 活性。结果表明，HO 对精子参数有不良影响，降低了精子活力和活力，同时增加了氧化应激，表现为脂质过氧化和 caspase-3 活性升高。GYY4137 对 HO 诱导的氧化应激（OS）具有剂量依赖性的保护作用。我们得出结论，在辅助生殖技术中体外精子准备过程中补充 GYY4137 可能提供抗氧化保护。
