Laboratory of Key Technology and Materials in Minimally Invasive Spine Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China.
Center for Spinal Minimally Invasive Research, Shanghai Jiao Tong University, Shanghai 200336, China.
ACS Appl Mater Interfaces. 2024 Nov 13;16(45):61664-61678. doi: 10.1021/acsami.4c13119. Epub 2024 Oct 30.
The management of critical-sized bone defects poses significant clinical challenges, particularly in the battlefield and trauma-related injuries. However, bone tissue engineering scaffolds that satisfy high porosity and good angiogenic and osteogenic functions are scarce. In this study, 3D nanofiber scaffolds decorated with strontium nanoparticles (3DS-Sr) were fabricated by combining electrospinning and gas foaming. Sodium borohydride (NaBH) served a dual role as both a reducing and gas-foaming agent, enabling a one-step process for expansion and modification. experimental results demonstrated that 3DS-Sr possessed an integrated multilayered porous structure. It promoted angiogenesis by upregulating the expression of hypoxia-inducible factor-1α (HIF-1α) protein and phosphorylation of ERK through the sustained release of Sr and created a favorable microenvironment for osteogenesis by activating the Wnt/β-catenin pathway. experiments indicated that 3DS-Sr promoted cranial bone regeneration by synergistically promoting the effects of vascularization and osteogenesis. In summary, this study proposed a bioactive bone scaffold in a "one stone, two birds" manner, providing a promising strategy for bone defect repair.
治疗临界尺寸骨缺损具有重要的临床意义，特别是在战场和创伤相关的损伤中。然而，满足高孔隙率和良好的血管生成及成骨功能的骨组织工程支架仍然稀缺。在这项研究中，通过静电纺丝和气体发泡相结合，制备了负载锶纳米粒子的 3D 纳米纤维支架（3DS-Sr）。硼氢化钠（NaBH）同时作为还原剂和气体发泡剂，实现了膨胀和修饰的一步法过程。实验结果表明，3DS-Sr 具有集成的多层多孔结构。它通过持续释放 Sr 来上调缺氧诱导因子-1α（HIF-1α）蛋白的表达和 ERK 的磷酸化，促进血管生成，并通过激活 Wnt/β-连环蛋白通路为成骨作用创造有利的微环境。实验表明，3DS-Sr 通过协同促进血管生成和成骨作用来促进颅骨再生。总之，本研究以“一石二鸟”的方式提出了一种具有生物活性的骨支架，为骨缺损修复提供了一种有前途的策略。
