Programa de Pós-graduação em Ciência Animal - PPGCA, Universidade Estadual do Maranhão - UEMA, São Luís, MA, Brasil.
Imunodot Diagnósticos Veterinários - IMUNODOT, Jaboticabal, SP, Brasil.
Rev Bras Parasitol Vet. 2024 Oct 28;33(4):e003424. doi: 10.1590/S1984-29612024066. eCollection 2024.
The aim of this study was to detect trypomastigote forms of Trypanosoma vivax, in blood smears, DNA of T. vivax and anti-T. vivax antibodies in samples from buffalos reared in the lowlands of Maranhão, Brazil. Blood samples were collected from 116 buffalos and 25 ectoparasite specimens. Blood smears were produced to diagnose forms compatible with Trypanosoma spp.; the indirect enzyme-linked immunosorbent assay (iELISA) and lateral-flow immunochromatography (Imunotest®) serological tests were used; and the polymerase chain reaction (PCR) was used to make molecular diagnoses. No forms compatible with Trypanosoma spp. were observed in blood smears. Among the 116 serum samples analyzed, 79.31% and 76.72% were positive in the ELISA and rapid tests, respectively. One sample was positive in the molecular test. Twenty-five lice of the species Haematopinus tuberculatus were collected. When subjected to PCR for detection of DNA of T. vivax, all of them were negative. The louse specimens were negative for T. vivax. There were no statistically significant differences (p < 0.05) in the presence of T. vivax in this region, in relation to the animals' age and sex. It can be concluded that these protozoa are circulating in the buffalo herd of the lowlands of Maranhão displaying crypitc parasitemias.
本研究旨在检测巴西马拉尼昂低地养殖的水牛血液涂片、T. vivax DNA 和抗 T. vivax 抗体中的锥虫形式。从 116 头水牛和 25 个外寄生虫标本中采集了血液样本。制作血涂片以诊断与锥虫属相符的形式；使用间接酶联免疫吸附试验（iELISA）和侧向流动免疫层析（Imunotest®）血清学检测；并使用聚合酶链反应（PCR）进行分子诊断。血涂片未观察到与锥虫属相符的形式。在分析的 116 份血清样本中，ELISA 和快速检测的阳性率分别为 79.31%和 76.72%。一份样本在分子检测中呈阳性。共采集了 25 只 Haematopinus tuberculatus 虱子。对 T. vivax DNA 的 PCR 检测结果均为阴性。虱子标本未检出 T. vivax。在该地区，与动物的年龄和性别无关，T. vivax 的存在没有统计学差异（p < 0.05）。可以得出结论，这些原生动物在马拉尼昂低地的水牛群中循环，表现出隐匿性寄生虫血症。
