Universidade Federal de Minas Gerais, Departamento de Genética, Ecologia e Evolução, Belo Horizonte, MG, Brasil.
Instituto Hermes Pardini-Grupo Fleury, Microbiologia, Núcleo de Operações Técnicas, Vespasiano, MG, Brasil.
Mem Inst Oswaldo Cruz. 2024 Oct 25;119:e240156. doi: 10.1590/0074-02760240156. eCollection 2024.
Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle.
The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle.
The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis.
The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin.
The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle.
犬小棒状杆菌是一种常从患有外耳炎或皮炎的犬中分离出来的细菌物种，也是一种通过狗咬伤传播的人畜共患病病原体。它被认为是一种机会性病原体，但对其致病机制的研究甚少。比较基因组学可以识别毒力和生态位因素，有助于了解其生活方式。
本项目的目的是比较犬小棒状杆菌的基因组，以确定与毒力和生活方式相关的基因。
使用 Illumina HiSeq 2500（Illumina，CA，USA）对 32 株进行测序，并使用 Unicycler 进行组装。还将分析来自 GenBank 中同一物种的另外两个非冗余基因组。所有基因组都进行了注释，并检查了分类学、组装质量、移动元件、CRISPR-Cas 系统以及毒力和抗微生物药物耐药性基因。将这三个基因组中的毒力基因与通常与犬小棒状杆菌一起分离的其他病原体的毒力基因进行了比较。
该物种有 42 个毒力因子，可以归类为生态位因子，因为在主要病原体中没有发现真正的毒力因子。基因 rbpA 可能赋予利福平的基础耐药水平。
三个基因组中缺乏真正的毒力因子表明犬小棒状杆菌具有机会性病原体的生活方式。
