Ma Jiage, Tan Zhongmei, Wu Mengguo, Tian Zihao, Xu Cong, Zhang Jing, Ma Yue, Feng Zhibiao, Yu Wei, Li Bailiang, Yao Yuchang, Jiang Zhanmei, Hou Juncai
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, PR China; College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, PR China.
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, PR China.
Int J Biol Macromol. 2024 Dec;282(Pt 3):136907. doi: 10.1016/j.ijbiomac.2024.136907. Epub 2024 Oct 28.
Polyvinyl alcohol (PVA)/fucoidan (FUC) blend nanofibers were systematically fabricated to co-encapsulate probiotic Lactiplantibacillus plantarum 69-2 (LP69-2) and four kinds of polyphenols by electrospinning for the first time. Scanning electron microscopy showed that some areas of PVA/FUC nanofibers encapsulated with LP69-2 were locally broadened. Attenuated total reflectance-Fourier transform infrared spectroscopy and X-ray diffraction suggested that LP69-2 and polyphenol were successfully encapsulated in PVA/FUC electrospun nanofibers. Thermogravimetric analysis revealed that the addition of LP69-2 and polyphenol enhanced the thermal stability of nanofibers. Moreover, the incorporation of FUC and polyphenol significantly increased the ABTS and DPPH radical scavenging ability of PVA nanofibers (P < 0.05). Notably, PVA/FUC/LP69-2/DMY nanofibers displayed the highest DPPH radical scavenging ability. After 21 d, these nanofibers loaded with polyphenols could maintain viability of LP69-2 over 7 lg CFU/g at 4 °C and the viability of LP69-2 in PVA/FUC/DMY nanofibers was the highest. Overall, the co-encapsulation of probiotic and polyphenol within PVA/FUC electrospun nanofibers increased the viability of probiotics and enhanced antioxidant activity of nanofibers. This study provided unique insights for protecting probiotics and developing novel functional foods with higher probiotics.
首次通过静电纺丝系统制备了聚乙烯醇（PVA）/岩藻依聚糖（FUC）共混纳米纤维，用于共包封益生菌植物乳杆菌69-2（LP69-2）和四种多酚。扫描电子显微镜显示，包裹LP69-2的PVA/FUC纳米纤维的某些区域局部变宽。衰减全反射傅里叶变换红外光谱和X射线衍射表明，LP69-2和多酚成功包封在PVA/FUC静电纺纳米纤维中。热重分析表明，LP69-2和多酚的加入提高了纳米纤维的热稳定性。此外，FUC和多酚的掺入显著提高了PVA纳米纤维的ABTS和DPPH自由基清除能力（P<0.05）。值得注意的是，PVA/FUC/LP69-2/DMY纳米纤维表现出最高的DPPH自由基清除能力。21天后，这些负载多酚的纳米纤维在4℃下可使LP69-2的活力维持在7 lg CFU/g以上，且PVA/FUC/DMY纳米纤维中LP69-2的活力最高。总体而言，在PVA/FUC静电纺纳米纤维中共包封益生菌和多酚提高了益生菌的活力，并增强了纳米纤维的抗氧化活性。本研究为保护益生菌和开发具有更高益生菌含量的新型功能性食品提供了独特的见解。
