Bioengineering Institute of Technology (BIT), Universitat Internacional de Catalunya (UIC), Barcelona, 08017, Spain.
Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Turkey; Institute of Pure and Applied Sciences, Marmara University, Department of Metallurgical and Materials Engineering, Istanbul, Turkey.
J Mech Behav Biomed Mater. 2023 Dec;148:106163. doi: 10.1016/j.jmbbm.2023.106163. Epub 2023 Oct 7.
The polymeric nanofiber may interact and control certain regeneration processes at the molecular level to repair damaged tissues. This research focuses on the development of characterization and antibacterial capabilities of polyvinyl alcohol (PVA)/chitosan (CS) nanofibres containing fucoidan (FUC) for tissue engineering as a skin tissue substitute. A control group consisting of 13% PVA/(0.1)% CS nanofiber was prepared. To confer antibacterial properties to the nanofiber, 10, 20, and 30 mg of FUC were incorporated into this control group. The scanning electron microscope (SEM) proved the homogeneous and beadless structures of the nanofibers. The antibacterial activity of the 13% PVA/(0.1)% CS/(10, 20, 30) FUC was tested against the S.aureus and E.coli and the results showed that with FUC addition, the antibacterial activities of the nanofibers increased. The biocompatibility test was performed with a fibroblast cell line for 1, 3, and 7 days of incubation and the results demonstrated that FUC addition enhanced the bioactivity of the 13% PVA/(0.1)% CS nanofibers. In addition, the biocompatibility results showed that 13% PVA/(0.1)% CS/10 FUC had the highest viability value for all incubation periods compared to the others. In addition, the tensile test results showed that; the maximum tensile strength value was observed for 13% PVA/(0.1)% CS/10 FUC nanofibers.
聚合物纳米纤维可以在分子水平上相互作用并控制某些再生过程，以修复受损组织。本研究专注于开发具有壳聚糖（CS）/聚乙烯醇（PVA）纳米纤维特征和抗菌能力的研究，其中包含岩藻聚糖硫酸酯（FUC），以作为组织工程的皮肤组织替代品。制备了包含 13% PVA/（0.1）% CS 纳米纤维的对照组。为了赋予纳米纤维抗菌性能，将 10、20 和 30mg 的 FUC 掺入该对照组中。扫描电子显微镜（SEM）证明了纳米纤维的均匀和无珠状结构。测试了 13% PVA/（0.1）% CS/（10、20、30）FUC 对金黄色葡萄球菌和大肠杆菌的抗菌活性，结果表明，随着 FUC 的加入，纳米纤维的抗菌活性增加。用成纤维细胞系进行了为期 1、3 和 7 天的孵育的生物相容性测试，结果表明 FUC 的加入增强了 13% PVA/（0.1）% CS 纳米纤维的生物活性。此外，生物相容性结果表明，与其他组相比，所有孵育期 13% PVA/（0.1）% CS/10 FUC 的细胞活力值最高。此外，拉伸试验结果表明，在所有拉伸试验中，13% PVA/（0.1）% CS/10 FUC 纳米纤维的最大拉伸强度值最高。
