College of Environment, Zhejiang University of Technology, Hangzhou, 310014, China; Zhejiang Ecology and Environment Group Co., Ltd., Hangzhou, 311100, China.
College of Environment, Zhejiang University of Technology, Hangzhou, 310014, China.
Environ Pollut. 2024 Dec 15;363(Pt 2):125216. doi: 10.1016/j.envpol.2024.125216. Epub 2024 Oct 28.
As a typical sulfur-containing volatile organic compound, dimethyl disulfide (DMDS) is known for its high toxicity and resistance to degradation, necessitating efficient control in environmental media. To address the limitations of biological treatment in degradation capacity, this study employs electro-stimulation to promote DMDS elimination by a porous polyaniline@carbon nanotube bioanode developed on graphite sheet (PANI@CNT/GS). Compared with the unmodified GS bioanode, the PANI@CNT/GS bioanode demonstrates significant advantages in biofilm activity, redox property, and DMDS degradation efficiency. Kinetics analysis shows that the maximum degradation rate of the PANI@CNT/GS bioanode was 0.60 mM h, which is 1.36 times higher than that of the control. Characterization results reveal that the highly active biofilms in PANI@CNT/GS bioanode possess 1.40 times the amount of living cells and a 12.5% increase in thickness, contributing to the notable enhancement in DMDS degradation capacity. Additionally, functional gene annotation indicates that the PANI@CNT/GS electrode facilitates the motility and activity of microbial cells and enriches the genes encoding key enzymes involved in DMDS metabolism. This work validates the feasibility of electro-stimulation for enhancing DMDS degradation and further provides in-depth insights into the process intensification mechanism from the perspectives of biofilm spatial structure and key functional genes.
作为一种典型的含硫挥发性有机化合物，二甲基二硫（DMDS）因其高毒性和抗降解性而备受关注，因此需要在环境介质中进行有效的控制。为了解决生物处理在降解能力方面的局限性，本研究采用电刺激技术，通过在石墨片上开发的多孔聚苯胺@碳纳米管生物阳极（PANI@CNT/GS）来促进 DMDS 的消除。与未修饰的 GS 生物阳极相比，PANI@CNT/GS 生物阳极在生物膜活性、氧化还原性能和 DMDS 降解效率方面具有显著优势。动力学分析表明，PANI@CNT/GS 生物阳极的最大降解速率为 0.60 mM h，是对照的 1.36 倍。表征结果表明，PANI@CNT/GS 生物阳极中高活性生物膜的活细胞数量增加了 1.40 倍，厚度增加了 12.5%，这有助于显著提高 DMDS 的降解能力。此外，功能基因注释表明，PANI@CNT/GS 电极促进了微生物细胞的运动和活性，并丰富了编码 DMDS 代谢关键酶的基因。这项工作验证了电刺激增强 DMDS 降解的可行性，并从生物膜空间结构和关键功能基因的角度深入探讨了过程强化的机制。
