Zhao Jingkai, Rao Manli, Zhang Hanyu, Wang Qinlin, Shen Yao, Ye Jiexu, Feng Ke, Zhang Shihan
Zhejiang Key Laboratory of Clean Energy Conversion and Utilization, Science and Education Integration College of Energy and Carbon Neutralization, Zhejiang University of Technology, Hangzhou 310014, China; College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
Water Res. 2025 Apr 15;274:123071. doi: 10.1016/j.watres.2024.123071. Epub 2024 Dec 30.
This study proposes a switching operating mode that alternates between microbial fuel cell (MFC) and microbial electrolysis cell (MEC) to restore the biofilm activity and organic pollutant degradation capacity in bioelectrochemical systems (BESs) during prolonged operation. After the model switching, the toluene degradation kinetics in BESs equipped with graphite sheet (GS) and polyaniline@carbon nanotubes (PANI@CNTs) bioanodes were elevated by 2.10 and 3.14 times, respectively. Nevertheless, the amount of active biomass in the GS and PANI@CNTs bioanodes only increased by 1.04 and 1.05 times, with the PANI@CNTs bioanode consistently outperforming in hierarchical biofilm activity and redox properties. Additionally, the distribution of functional genes across the dominant genera revealed their roles in extracellular electron transfer and the four steps of toluene degradation (primary oxidation, ring-opening, intermediate oxidation, and tricarboxylic acid cycle). Furthermore, the cooperation of substrate exchange among Pseudomonas, Alicycliphilus, and Acidovorax in the MFC mode evolved to interactions among Acidovorax, Alicycliphilus, and Geobacter in the MEC mode, which attributed to the nonlinear relationship between active biomass and pollutant degradation capacity. These results provide insights into the operating mode and interspecific interactions of BESs, with implications for practical applications.
本研究提出了一种在微生物燃料电池（MFC）和微生物电解池（MEC）之间交替的切换运行模式，以恢复生物电化学系统（BESs）在长期运行期间的生物膜活性和有机污染物降解能力。模式切换后，配备石墨片（GS）和聚苯胺@碳纳米管（PANI@CNTs）生物阳极的BESs中甲苯降解动力学分别提高了2.10倍和3.14倍。然而，GS和PANI@CNTs生物阳极中活性生物量仅分别增加了1.04倍和1.05倍，其中PANI@CNTs生物阳极在分层生物膜活性和氧化还原特性方面始终表现更优。此外，优势菌属中功能基因的分布揭示了它们在细胞外电子转移以及甲苯降解的四个步骤（初级氧化、开环、中间氧化和三羧酸循环）中的作用。此外，MFC模式下假单胞菌、嗜环脂菌和嗜酸菌之间的底物交换协作演变为MEC模式下嗜酸菌、嗜环脂菌和地杆菌之间的相互作用，这归因于活性生物量与污染物降解能力之间的非线性关系。这些结果为BESs的运行模式和种间相互作用提供了见解，对实际应用具有启示意义。
