Li Anyang, Chen Chen, Li Yanmei, Wang Yanshuang, Li Xuemiao, Zhu Qiao, Zhang Yue, Tian Shen, Xia Qianfeng
NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine and the Second Affiliated Hospital, Hainan Medical University, Haikou, Hainan, China.
Medical Laboratory Department, Traditional Chinese Medicine Hospital of Yaan, Sichuan, China.
Res Microbiol. 2025 Jan-Feb;176(1-2):104250. doi: 10.1016/j.resmic.2024.104250. Epub 2024 Oct 29.
Multidrug-resistant (MDR) Pseudomonas aeruginosa is a serious life-threatening pathogen. The rise in P. aeruginosa resistance rates has renewed interest in phages as an alternative therapeutic approach for treating bacterial infections. In this study, we investigated the characteristics of the first Pseudomonas phage, vB_PaP_HN01, isolated from Hainan, the only tropical island in China. The lytic rate of this phage against P. aeruginosa reached 64.3 % (27/42). Under the optimal multiplicity of infection (MOI) of 0.1, more than 90 % of phage particles absorb onto the host cell within 10 min, with an eclipse period of around 15 min, and a high titer phage production (10 PFU/ml) within 90 min was demonstrated. vB_PaP_HN01 maintains a robust titer after 1 h exposure to pH values and temperatures (up to 50 °C). Genome annotation revealed that vB_PaP_HN01 did not contain drug-resistance or lysogeny-associated genes. It can effectively inhibit the formation of biofilms of MDR P. aeruginosa and eliminated aggressive biofilms (removal rate about 70 %). In the in vivo infection models, it was demonstrated that the survival rate and lifespan of Galleria mellonella larvae were increased alongside the injection of vB_PaP_HN01. These data revealed the potential of vB_PaP_HN01 against P. aeruginosa in clinic.
多重耐药（MDR）铜绿假单胞菌是一种严重威胁生命的病原体。铜绿假单胞菌耐药率的上升使人们重新对噬菌体作为治疗细菌感染的替代治疗方法产生兴趣。在本研究中，我们调查了从中国唯一的热带岛屿海南分离出的首个铜绿假单胞菌噬菌体vB_PaP_HN01的特性。该噬菌体对铜绿假单胞菌的裂解率达到64.3%（27/42）。在最佳感染复数（MOI）为0.1的情况下，超过90%的噬菌体颗粒在10分钟内吸附到宿主细胞上，潜伏期约为15分钟，并在90分钟内产生高滴度噬菌体（10⁹ PFU/ml）。vB_PaP_HN01在暴露于pH值和温度（高达50°C）1小时后仍保持较高滴度。基因组注释显示，vB_PaP_HN01不包含耐药或溶原相关基因。它可以有效抑制多重耐药铜绿假单胞菌生物膜的形成，并消除成熟生物膜（去除率约70%）。在体内感染模型中，结果表明，随着vB_PaP_HN01的注射，大蜡螟幼虫的存活率和寿命增加。这些数据揭示了vB_PaP_HN01在临床上对抗铜绿假单胞菌的潜力。
