松籽壳堿性提取物的潜在药用功效：抗 UVC 活性和巨噬细胞活化。
Potential Medicinal Efficacy of Alkaline Extract of Pine Seed Shell: Anti-UVC Activity and Macrophage Activation.
机构信息
Meikai University Research Institute of Odontology (M-RIO), Saitama, Japan.
Faculty of Dentistry, Autonomous University of the State of Mexico (UAEMex), Toluca, Mexico.
出版信息
In Vivo. 2024 Nov-Dec;38(6):2629-2638. doi: 10.21873/invivo.13739.
BACKGROUND/AIM: Alkaline extracts of several plants which contain lignin degradation products have several unique biological activities. In order to search for new biological activities of alkaline extracts of pine seed shell (APs), their anti-ultraviolet C (UVC) and macrophage stimulation activity were investigated.
MATERIALS AND METHODS
Anti-UVC activity was determined by the ratio of the 50% cytotoxic concentration against human melanoma cell line COLO679 to the 50% UVC-protective concentration. Extracellularly secreted nitrite (NO ) by unstimulated and lipopolysaccharide (LPS)-stimulated mouse macrophage-like cells RAW264.7 was determined by Griess method.
RESULTS
APs showed significantly higher anti-UVC activity than previously reported hot-water extracts of medical herbs. Anti-UVC activity of AP and vanillic acid was maintained for much longer than that of sodium ascorbate and vanillin. APs enhanced the production of NO to the level induced by LPS. Simultaneous addition of AP and LPS did not further increase NO production, suggesting their mechanisms of action overlap.
CONCLUSION
The present study suggests the possible application of APs as UVC protectors and immunopotentiators via macrophage activation.
背景/目的：几种含有木质素降解产物的植物的碱性提取物具有多种独特的生物学活性。为了寻找松仁壳碱性提取物（APs）的新的生物学活性，研究了其抗紫外线 C（UVC）和巨噬细胞刺激活性。
材料和方法
通过比较对人黑素瘤细胞系 COLO679 的 50%细胞毒性浓度与 50%UVC 保护浓度的比值，来确定抗 UVC 活性。通过格里斯法测定未刺激和脂多糖（LPS）刺激的小鼠巨噬细胞样细胞 RAW264.7 细胞分泌的细胞外亚硝酸盐（NO ）。
结果
与之前报道的草药热水提取物相比，APs 表现出显著更高的抗 UVC 活性。AP 和香草酸的抗 UVC 活性比抗坏血酸钠和香草醛维持的时间长得多。APs 增强了 NO 的产生，使其达到 LPS 诱导的水平。同时添加 APs 和 LPS 并没有进一步增加 NO 的产生，这表明它们的作用机制有重叠。
结论
本研究表明，APs 可能通过激活巨噬细胞作为 UVC 保护剂和免疫增强剂应用。
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