Cardiovascular Research Group, Khon Kaen University, Khon Kaen, Thailand.
School of Medical Technology, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
In Vivo. 2024 Nov-Dec;38(6):3033-3040. doi: 10.21873/invivo.13787.
BACKGROUND/AIM: Atherosclerosis is a vascular inflammatory disease characterized by the activation and stress of various inflammatory cells, leading to the development of coronary artery disease and subsequently acute myocardial infarction (AMI). Among AMI cases, ST-segment elevation myocardial infarction (STEMI) is typically more severe than non-STEMI (NSTEMI). UL16-binding proteins (ULBPs), which are NKG2D ligands, can be expressed on the surface of stressed and activated cells, prompting these cells to generate microparticles (MPs). Consequently, MPs carrying ULBPs, particularly ULBP1 (ULBP1 MPs), may be released into the bloodstream. This study aimed to investigate the association between ULBP1 MPs and the presence of AMI and its severity.
We recruited 58 AMI patients and 45 age-matched control subjects. Levels of ULBP1 MPs and ULBP1 MPs originating from T lymphocytes (ULBP1 TMPs) were measured using flow cytometry.
Both ULBP1 MP and ULBP1 TMP levels were significantly elevated in AMI patients compared to controls. Elevated levels of these MPs were independent risk factors for AMI with odds ratios (OR) of 4.3 (95%CI=1.5-12.3) for ULBP1 MPs and 5.8 (95%CI=2.0-17.0) for ULBP1 TMPs. Additionally, ULBP1 TMP levels were significantly higher in STEMI patients compared to NSTEMI patients, with an independent association observed between ULBP1 TMPs and STEMI (OR=3.9; 95%CI=1.2-12.8).
Elevated levels of ULBP1 MPs and ULBP1 TMPs are associated with AMI and its severity. These biomarkers could serve as indicators of vulnerable plaques that lead to AMI.
背景/目的：动脉粥样硬化是一种血管炎症性疾病，其特征为各种炎症细胞的激活和应激，导致冠状动脉疾病的发展，并随后引发急性心肌梗死（AMI）。在 AMI 病例中，ST 段抬高型心肌梗死（STEMI）通常比非 ST 段抬高型心肌梗死（NSTEMI）更为严重。UL16 结合蛋白（ULBPs）是 NKG2D 配体，可在应激和激活的细胞表面表达，促使这些细胞产生微颗粒（MPs）。因此，带有 ULBPs 的 MPs，特别是 ULBP1（ULBP1 MPs），可能会释放到血液中。本研究旨在探讨 ULBP1 MPs 与 AMI 及其严重程度的关系。
我们招募了 58 名 AMI 患者和 45 名年龄匹配的对照者。使用流式细胞术测量 ULBP1 MPs 和源自 T 淋巴细胞的 ULBP1 MPs（ULBP1 TMPs）的水平。
与对照组相比，AMI 患者的 ULBP1 MP 和 ULBP1 TMP 水平均显著升高。这些 MPs 水平升高是 AMI 的独立危险因素，ULBP1 MPs 的优势比（OR）为 4.3（95%CI=1.5-12.3），ULBP1 TMPs 的 OR 为 5.8（95%CI=2.0-17.0）。此外，STEMI 患者的 ULBP1 TMP 水平明显高于 NSTEMI 患者，ULBP1 TMP 与 STEMI 之间存在独立关联（OR=3.9；95%CI=1.2-12.8）。
ULBP1 MPs 和 ULBP1 TMPs 水平升高与 AMI 及其严重程度相关。这些生物标志物可作为导致 AMI 的易损斑块的指标。
