Haohan Songpol, Pussadhamma Burabha, Jumnainsong Amonrat, Leuangwatthananon Wit, Makarawate Pattarapong, Leelayuwat Chanvit, Komanasin Nantarat
Cardiovascular Research Group, Khon Kaen University, Khon Kaen 40002, Thailand.
Biomedical Sciences Program, Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
Diagnostics (Basel). 2020 Sep 29;10(10):766. doi: 10.3390/diagnostics10100766.
Various cell types undergo activation and stress during atherosclerosis resulting in the development of acute myocardial infarction (AMI) in coronary artery disease (CAD). Major histocompatibility complex class I related chain A and B (MICA/B) can be expressed on the surface of activated and stressed cells and released into blood circulation in several forms including microparticles (MICA/B MPs) from various cell types. We aimed to investigate the association of these MICA/B MPs with the presence of AMI. Fifty-one AMI and 46 age-matched control subjects were recruited.
Levels of MICA/B MPs derived from various parent cells including endothelial cells, platelets, monocytes, neutrophils, and T lymphocytes were determined by flow cytometry.
The levels and proportion of MICA/B MPs from all types of cell origin were significantly increased in AMI patients compared to those of the controls. A multivariate regression model showed an independent association between MICA/B MPs and AMI (OR = 11.6; 95% CI = 2.8, 47.3). Interestingly, based on the disease severity, we found that the levels of MICA/B MPs were significantly elevated in the ST-segment elevation myocardial infarction (STEMI) compared to the non-STEMI (NSTEMI) patients. Moreover, an independent association of MICA/B MPs with the occurrence of STEMI was also demonstrated (OR = 4.1; 95% CI = 1.5, 16.7).
These results suggest that MICA/B MPs are associated with AMI and disease severity. They may act as mediators contributing to the pathological process of AMI. Alternatively, they are the results of various cell activations contributing to AMI.
在动脉粥样硬化过程中，多种细胞类型会发生激活和应激反应，进而导致冠状动脉疾病（CAD）患者发生急性心肌梗死（AMI）。主要组织相容性复合体I类相关链A和B（MICA/B）可在激活和应激的细胞表面表达，并以多种形式释放到血液循环中，包括来自各种细胞类型的微粒（MICA/B MPs）。我们旨在研究这些MICA/B MPs与AMI发生之间的关联。招募了51例AMI患者和46例年龄匹配的对照受试者。
通过流式细胞术测定来自包括内皮细胞、血小板、单核细胞、中性粒细胞和T淋巴细胞在内的各种亲本细胞的MICA/B MPs水平。
与对照组相比，AMI患者中所有细胞来源的MICA/B MPs水平和比例均显著升高。多变量回归模型显示MICA/B MPs与AMI之间存在独立关联（OR = 11.6；95% CI = 2.8，47.3）。有趣的是，根据疾病严重程度，我们发现与非ST段抬高型心肌梗死（NSTEMI）患者相比，ST段抬高型心肌梗死（STEMI）患者的MICA/B MPs水平显著升高。此外，还证实了MICA/B MPs与STEMI的发生存在独立关联（OR = 4.1；95% CI = 1.5，16.7）。
这些结果表明MICA/B MPs与AMI及其疾病严重程度相关。它们可能作为介质促成AMI的病理过程。或者，它们是促成AMI的各种细胞激活的结果。
