Gonzales Clarice F, Cowling Craig L, Kelley Dior R
Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011, USA.
Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50011, USA
Cold Spring Harb Protoc. 2024 Oct 30. doi: 10.1101/pdb.prot108623.
The rolled towel assay (RTA) is a soil-free method to evaluate juvenile phenotypes in crops such as maize and soybean. Here, we provide an updated RTA-based protocol to phenotype maize seedling responses to chemicals of interest. We exemplify the protocol with two synthetic auxin herbicides (2,4-dichlorophenoxyacetic acid and picloram), an auxin precursor (indole-3-butyric acid), and an auxin inhibitor (-1-naphthylphthalamic acid), but the method can be used with other hormones or plant growth regulators that are soluble in growth media. We also include instructions on how to annotate root traits and analyze primary root length trait data. The protocol can be scaled up for use in genetic screens, preparing tissue for gene expression analyses, carrying out genome-wide association studies (GWASs), and quantitative trait locus (QTL) identification.
卷纸试验（RTA）是一种无需土壤的方法，用于评估玉米和大豆等作物的幼苗表型。在此，我们提供了一种基于RTA的更新方案，用于对玉米幼苗对感兴趣化学物质的反应进行表型分析。我们以两种合成生长素除草剂（2,4-二氯苯氧乙酸和毒莠定）、一种生长素前体（吲哚-3-丁酸）和一种生长素抑制剂（N-1-萘基邻苯二甲酸）为例来说明该方案，但该方法可用于其他可溶于生长培养基的激素或植物生长调节剂。我们还包括了如何注释根系性状以及分析初生根长度性状数据的说明。该方案可扩大规模用于遗传筛选、为基因表达分析准备组织、进行全基因组关联研究（GWAS）以及鉴定数量性状基因座（QTL）。
