利用铝佐剂-海藻酸钠增强 RBD-FC 的免疫原性以对抗 SARS-COV-2。

Improvement of RBD-FC Immunogenicity by Using Alum-Sodium Alginate Adjuvant Against SARS-COV-2.

机构信息

Department of Cellular and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Protein Research Center, Shahid Beheshti University, Tehran, Iran.

出版信息

Influenza Other Respir Viruses. 2024 Nov;18(11):e70018. doi: 10.1111/irv.70018.

DOI:10.1111/irv.70018

PMID:39478310

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11525037/

Abstract

BACKGROUND

Adjuvants use several mechanisms to boost immunogenicity and to modulate immune response. The strength of adsorption of antigen by adjuvants can be a determinant factor for significant improvement of immunopotentiation.

METHODS

We expressed recombinant RBD-FC in PichiaPink Strain 4 and examined the vaccination of mice by vaccine formulation with different adjuvants (sodium alginate and aluminum hydroxide, alone and together).

RESULTS

Sodium alginate significantly increased the immunogenicity and stability of RBD-FC antigen, so RBD-FC formulated with combined alginate and alum (AlSa) and sodium alginate alone showed higher antibody titer and stability. Immunogenicity of RBD-FC:AlSa was determined by serological assays including direct enzyme-linked immunosorbent assay (ELISA) and surrogate virus neutralization test (sVNT). High levels of IgGs and neutralizing antibodies were measured in serum of mice immunized with the RBD-FC:AlSa formulation. On the other hand, cytokines IL-10 and INF-γ were severely accumulated in response to RBD-FC:AlSa, and after 10 days, their accumulation was significantly declined, whereas IL-4 showed the highest and the lowest accumulation in response to alum and alginate, respectively.

CONCLUSIONS

Our data may suggest that combination of alum and sodium alginate has a better compatibility with RBD-FC in vaccine formulation.

摘要

背景

佐剂通过多种机制增强免疫原性并调节免疫反应。佐剂对抗原的吸附强度可能是显著增强免疫的决定因素。

方法

我们在毕赤酵母Pink 菌株 4 中表达了重组 RBD-FC，并通过用不同佐剂（藻酸钠和氢氧化铝，单独和组合）配制的疫苗来检查小鼠的疫苗接种。

结果

藻酸钠显著提高了 RBD-FC 抗原的免疫原性和稳定性，因此 RBD-FC 与藻酸钠和明矾（AlSa）组合以及单独的藻酸钠配制的疫苗显示出更高的抗体滴度和稳定性。RBD-FC 的免疫原性：通过血清学检测包括直接酶联免疫吸附测定（ELISA）和替代病毒中和试验（sVNT）来确定。用 RBD-FC：AlSa 制剂免疫的小鼠血清中测量到高水平的 IgG 和中和抗体。另一方面，细胞因子 IL-10 和 INF-γ在对 RBD-FC：AlSa 的反应中严重积累，并且在 10 天后，其积累明显下降，而 IL-4 对明矾和藻酸钠的反应分别显示出最高和最低的积累。