Cano-Vicent Alba, Hashimoto Rina, Takayama Kazuo, Serrano-Aroca Ángel
Biomaterials and Bioengineering Laboratory, Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia San Vicente Mártir, c/Guillem de Castro 94, 46001 Valencia, Spain.
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.
Polymers (Basel). 2022 Apr 6;14(7):1483. doi: 10.3390/polym14071483.
The current pandemic is urgently demanding the development of alternative materials capable of inactivating the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the coronavirus 2019 (COVID-19) disease. Calcium alginate is a crosslinked hydrophilic biopolymer with an immense range of biomedical applications due to its excellent chemical, physical, and biological properties. In this study, the cytotoxicity and antiviral activity of calcium alginate in the form of films were studied. The results showed that these films, prepared by solvent casting and subsequent crosslinking with calcium cations, are biocompatible in human keratinocytes and are capable of inactivating enveloped viruses such as bacteriophage phi 6 with a 1.43-log reduction (94.92% viral inactivation) and SARS-CoV-2 Delta variant with a 1.64-log reduction (96.94% viral inactivation) in virus titers. The antiviral activity of these calcium alginate films can be attributed to its compacted negative charges that may bind to viral envelopes inactivating membrane receptors.
当前的大流行迫切需要开发能够灭活导致2019冠状病毒病（COVID-19）的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的替代材料。海藻酸钙是一种交联的亲水性生物聚合物，由于其优异的化学、物理和生物学特性，在生物医学领域有广泛的应用。在本研究中，对薄膜形式的海藻酸钙的细胞毒性和抗病毒活性进行了研究。结果表明，这些通过溶液浇铸和随后与钙阳离子交联制备的薄膜在人角质形成细胞中具有生物相容性，并且能够灭活包膜病毒，如噬菌体phi 6，病毒滴度降低1.43个对数（病毒灭活率94.92%），以及SARS-CoV-2 Delta变异株，病毒滴度降低1.64个对数（病毒灭活率96.94%）。这些海藻酸钙薄膜的抗病毒活性可归因于其紧密的负电荷，这些负电荷可能与病毒包膜结合，从而使膜受体失活。
