Department of Plant Breeding, Justus Liebig University Giessen, Giessen, Germany.
Institute for Resistance Research and Stress Tolerance, Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Quedlinburg, Germany.
BMC Plant Biol. 2024 Oct 30;24(1):1032. doi: 10.1186/s12870-024-05692-3.
Precision phenotyping of short-term transpiration response to environmental conditions and transpiration patterns throughout wheat development enables a better understanding of specific trait compositions that lead to improved transpiration efficiency. Transpiration and related traits were evaluated in a set of 79 winter wheat lines using the custom-built "DroughtSpotter XXL" facility. The 120 l plant growth containers implemented in this phenotyping platform enable gravimetric quantification of water use in real-time under semi-controlled, yet field-like conditions across the entire crop life cycle.
The resulting high-resolution data enabled identification of significant developmental stage-specific variation for genotype rankings in transpiration efficiency. In addition, for all examined genotypes we identified the genotype-specific breakpoint in transpiration in response to increasing vapour pressure deficit, with breakpoints ranging between 2.75 and 4.1 kPa.
Continuous monitoring of transpiration efficiency and diurnal transpiration patterns enables identification of hidden, heritable genotypic variation for transpiration traits relevant for wheat under drought stress. Since the unique experimental setup mimics field-like growth conditions, the results of this study have good transferability to field conditions.
对短期蒸腾响应环境条件和整个小麦发育过程中蒸腾模式的精确表型分析，使人们能够更好地理解导致蒸腾效率提高的特定特征组合。使用定制的“DroughtSpotter XXL”设施，对 79 个冬小麦品系进行了蒸腾和相关性状的评估。该表型平台中实施的 120 升植物生长容器，可在整个作物生命周期中，在半控制但类似田间的条件下，实时对水的使用进行称重定量。
由此产生的高分辨率数据，能够确定蒸腾效率的基因型排名在发育阶段上的显著差异。此外，对于所有检查的基因型，我们确定了蒸腾对蒸气压亏缺增加的特定基因型断点，断点范围在 2.75 和 4.1kPa 之间。
持续监测蒸腾效率和日蒸腾模式，可以鉴定与干旱胁迫下小麦有关的蒸腾特性的隐性、可遗传的基因型变异。由于独特的实验设置模拟了类似田间的生长条件，因此本研究的结果具有很好的田间条件可转移性。
