Tytik Dmitrii L, Kuzmin Viktor I, Souvorova Olga V, Revina Alexandra A
Frumkin Institute of Physical Chemistry and Electrochemistry Russian Academy of Sciences (IPCE RAS), Moscow, Russia.
Russian Technological University (MIREA), Moscow, Russia.
Front Chem. 2024 Oct 16;12:1439250. doi: 10.3389/fchem.2024.1439250. eCollection 2024.
The article discusses optical properties (luminescence) of diluted (24 dilution factor) lucigenin () aqueous solutions. Six series of aqueous solutions, with 50 samples in each series, were studied. The series were diluted on different days within random schedules, following a unified procedure: the first sample in all the series was the (C = 8.2 × 10 mol/L) stock solution, while the rest of the samples were obtained by successive dilution with the ratio of 24. For the first three samples, the luminescence intensity decrease appropriately complied with the exponential function model (the dilution ratio: none for the stock solution, for the second and the third, 24 and 24 × 24 = 24, respectively). Starting from the fourth sample for statistical processing of luminescence data, the seven largest values were selected from the built rank distribution of emission intensity values. This method helps eliminate the influence of "random large bounces" when calculating the correlation coefficient. Up to the 50th studied sample, a challenging linear gradual decrease in the intensity of recorded photometric values was noted (correlation coefficients for all series being close to -0.9). Similar analysis of six reference series of pure water "dilution" samples did not exhibit any correlation between the highest emission values in the studied wavelength range (specific for bandwidth, 480-505 nm) and the sample's dilution number. It can be assumed that photometric values, recorded in the series of Lc sequentially diluted aqueous solutions after substance () elimination (theoretically expected after the 13th sample within the used experimental setup), could be attributed to the gradual destruction of long-lived aqueous structures formed in the process of hydration of molecules during its dissolution.
本文讨论了稀释（稀释因子为24）光泽精（）水溶液的光学性质（发光）。研究了六个系列的水溶液，每个系列有50个样品。这些系列在不同日期按照随机时间表进行稀释，遵循统一程序：所有系列的第一个样品是（C = 8.2×10⁻⁵ mol/L）储备溶液，而其余样品通过以24的比例连续稀释获得。对于前三个样品，光泽精发光强度的降低恰当地符合指数函数模型（稀释比例：储备溶液无，第二个和第三个分别为24和24×24 = 24²）。从第四个样品开始进行发光数据的统计处理时，从建立的发射强度值排名分布中选择七个最大值。这种方法有助于在计算相关系数时消除“随机大波动”的影响。直到第50个研究样品，记录的光度值强度出现了具有挑战性的线性逐渐下降（所有系列的相关系数接近-0.9）。对六个纯水“稀释”样品参考系列的类似分析表明，在所研究的波长范围（特定于带宽480 - 505 nm）内的最高发射值与样品的稀释倍数之间没有任何相关性。可以假设，在消除物质（）后（在所用实验装置中理论上在第13个样品之后预期），在依次稀释的光泽精水溶液系列中记录的光度值可能归因于在其溶解过程中光泽精分子水合过程中形成的长寿命水结构的逐渐破坏。
