Zhou Yuefan, Xu Yixin, Tian Tongguan, Xu Yanping
Nourse Centre for Pet Nutrition Wuhu China.
Food Sci Nutr. 2024 Aug 25;12(10):8200-8210. doi: 10.1002/fsn3.4404. eCollection 2024 Oct.
Hypertension significantly impacts the survival and quality of life of animals, often leading to chronic kidney failure. Current clinical drugs used to manage hypertension carry the risk of causing adverse reactions. In contrast, certain natural peptides have demonstrated the ability to safely reduce blood pressure by inhibiting the production of angiotensin. We administered four biologically active peptide solutions to spontaneously hypertensive rats: derived from corn, wheat, egg white, and soybean. The efficacy of these peptides in reducing blood pressure was assessed through regular measurements of systolic pressure. Additionally, we analyzed levels of angiotensin-converting enzyme and angiotensin 2 using immunohistochemistry and ELISA in vivo. The indicators of oxidative stress and inflammation in hypertensive rats were evaluated using qRT-PCR and ELISA, respectively. Both wheat (from 182.5 ± 12.26 mmHg at day 0 to 168.86 ± 5.86 mmHg at day 20, = .0435) and soybean (from 189 ± 2.19 mmHg at day 0 to 178.25 ± 5.14 mmHg at day 20, = .0017) notably lowered systolic blood pressure compared to their starting systolic blood pressures in spontaneously hypertensive rats. Both wheat and soybean peptides significantly reduced plasma ANG II levels, akin to captopril's effect. Wheat peptides additionally exhibited antioxidant properties. Only the corn peptide showed a significant increase in transcript levels of the proinflammatory factors IL-6 and TNF-α. At the protein level, all four kinds of peptides significantly elevated IL-6 levels while inhibiting TNF-α secretion. This study demonstrates that wheat peptides and soybean peptides administered as dietary supplements exhibit significant hypotensive and antioxidant effects.
高血压严重影响动物的生存和生活质量，常导致慢性肾衰竭。目前用于治疗高血压的临床药物存在引起不良反应的风险。相比之下，某些天然肽已证明能够通过抑制血管紧张素的产生来安全地降低血压。我们给自发性高血压大鼠施用了四种生物活性肽溶液：分别来源于玉米、小麦、蛋清和大豆。通过定期测量收缩压来评估这些肽在降低血压方面的功效。此外，我们在体内使用免疫组织化学和酶联免疫吸附测定法分析了血管紧张素转换酶和血管紧张素2的水平。分别使用定量逆转录聚合酶链反应和酶联免疫吸附测定法评估高血压大鼠的氧化应激和炎症指标。与自发性高血压大鼠的初始收缩压相比，小麦肽（从第0天的182.5±12.26毫米汞柱降至第20天的168.86±5.86毫米汞柱，P = 0.0435）和大豆肽（从第0天的189±2.19毫米汞柱降至第20天的178.25±5.14毫米汞柱，P = 0.0017）均显著降低了收缩压。小麦肽和大豆肽均显著降低了血浆血管紧张素II水平，其效果与卡托普利相似。小麦肽还具有抗氧化特性。只有玉米肽使促炎因子白细胞介素-6和肿瘤坏死因子-α的转录水平显著升高。在蛋白质水平上，所有四种肽均显著提高了白细胞介素-6水平，同时抑制了肿瘤坏死因子-α的分泌。本研究表明，作为膳食补充剂施用的小麦肽和大豆肽具有显著的降压和抗氧化作用。
