Tocharus Chainarong, Sutheerawattananonda Manote
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Foods. 2025 Apr 3;14(7):1256. doi: 10.3390/foods14071256.
Our previous research has shown that SDOs derived from yellow silk cocoons have hypotensive effects on rats in chronic toxicity testing. This study investigated the potential preventative and therapeutic benefits of SDOs on hypertensive rats induced by L-NAME. The experiment involved nine rat groups: (1) normal control, (2) normal + 200 mg kg-1 BW SDOs, (3) hypertensive (HT) control, (4) HT + 50 mg kg-1 BW SDOs, (5) HT + 100 mg kg-1 BW SDOs, (6) HT + 200 mg kg-1 BW SDOs, (7) HT + enalapril (Ena), (8) HT + soy protein isolate (SPI), and (9) HT + bovine serum albumin (BSA). In the preventative approach, rats received 40 mg kg-1 of L-NAME with the studied substances during the four-week investigation. SDOs given at doses of 100 and 200 mg kg-1 BW demonstrated a significant decrease in systolic blood pressure (SBP) without affecting heart rate (HR). In therapeutic studies, 40 mg kg-1 BW of L-NAME increased SBP in the experimental groups over the first four weeks, resulting in mean SBP values above 150 mmHg. Administering 100 and 200 mg kg-1 BW SDOs and 100 mg kg-1 BW SPI significantly reduced SBP. However, SDOs at 200 mg kg-1 BW exhibited SBP closer to the enalapril group. In functional vascular tests, rats given SDOs at a dose of 200 mg kg-1 BW had the highest relaxation and lowest contraction percentages, like the normal control groups. The research found that SDOs may inhibit and manage hypertension in both healthy and hypertensive rats by safeguarding endothelial cells.
我们之前的研究表明，从黄色蚕茧中提取的丝胶蛋白在慢性毒性试验中对大鼠有降压作用。本研究调查了丝胶蛋白对L-NAME诱导的高血压大鼠的潜在预防和治疗益处。实验涉及九个大鼠组：(1)正常对照组，(2)正常 + 200 mg kg-1体重丝胶蛋白组，(3)高血压（HT）对照组，(4) HT + 50 mg kg-1体重丝胶蛋白组，(5) HT + 100 mg kg-1体重丝胶蛋白组，(6) HT + 200 mg kg-1体重丝胶蛋白组，(7) HT + 依那普利（Ena）组，(8) HT + 大豆分离蛋白（SPI）组，以及(9) HT + 牛血清白蛋白（BSA）组。在预防方法中，大鼠在为期四周的研究期间接受40 mg kg-1的L-NAME与所研究的物质。给予100和200 mg kg-1体重剂量的丝胶蛋白可使收缩压（SBP）显著降低，而不影响心率（HR）。在治疗研究中，40 mg kg-1体重的L-NAME在实验的前四周使实验组的SBP升高，导致平均SBP值高于150 mmHg。给予100和200 mg kg-1体重的丝胶蛋白以及100 mg kg-1体重的SPI可显著降低SBP。然而，200 mg kg-1体重的丝胶蛋白组的SBP与依那普利组更接近。在功能性血管测试中，给予200 mg kg-1体重剂量丝胶蛋白的大鼠具有最高的舒张百分比和最低的收缩百分比，与正常对照组相似。研究发现，丝胶蛋白可能通过保护内皮细胞来抑制和控制健康大鼠和高血压大鼠的高血压。
