Li Wei-Ping, Bai Zi-Ran, Tian Yu-Qin, Yin Chun-Lai, Li Xia
Department of Immunology, College of Basic Medical Science, Dalian Medical University, Dalian 116044， Liaoning Province, China.
Department of Hematology, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2024 Oct;32(5):1518-1523. doi: 10.19746/j.cnki.issn.1009-2137.2024.05.033.
To investigate the frequencies and subset distribution of peripheral helper (Tph) T cells in patients with immune thrombocytopenia (ITP), and explore the pathogenesis of ITP.
A total of 25 newly diagnosed ITP patients treated in The Second Affiliated Hospital of Dalian Medical University from January to December 2022 were selected, and 25 healthy volunteers (age- and sex-matched) were recruited as the control group. Flow cytometry was used to detect the subsets of CD4 T cells and Tph cells.
The frequency of effector memory (CCR7 CD45ROCD4) T cells in ITP patients was significantly higher than that in healthy controls( < 0.05). The frequency of Tph cells in ITP patients was also significantly higher than that in healthy controls ( < 0.001), and most of the Tph cells in ITP patients were effector memory T cells. Furthermore, the expressions of T-cell costimulatory molecules in Tph cells, including ICOS and CD84, were similar to those in follicular helper T(Tfh) cells. CXCR3 CCR6 Tph (Tph2) subgroup was dominant in Tph cells, but the frequency of CXCR3CCR6 Tph (Tph1) cells in ITP patients was much higher than that in healthy controls ( < 0.05).
Tph cells, especially Tph1 cells, were abnormally expanded in ITP patients, which may be a potential etiology of ITP.
探讨免疫性血小板减少症（ITP）患者外周辅助性T细胞（Tph）的频率及亚群分布，以探索ITP的发病机制。
选取2022年1月至12月在大连医科大学附属第二医院治疗的25例新诊断ITP患者，并招募25名年龄和性别匹配的健康志愿者作为对照组。采用流式细胞术检测CD4 T细胞和Tph细胞亚群。
ITP患者中效应记忆（CCR7-CD45RO+CD4+）T细胞的频率显著高于健康对照组（P<0.05）。ITP患者中Tph细胞的频率也显著高于健康对照组（P<0.001），且ITP患者中的大多数Tph细胞为效应记忆T细胞。此外，Tph细胞中T细胞共刺激分子（包括ICOS和CD84）的表达与滤泡辅助性T（Tfh）细胞中的表达相似。CXCR3-CCR6-Tph（Tph2）亚群在Tph细胞中占主导地位，但ITP患者中CXCR3+CCR6-Tph（Tph1）细胞的频率远高于健康对照组（P<0.05）。
ITP患者中Tph细胞，尤其是Tph1细胞异常扩增，这可能是ITP的潜在病因。
