Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Clin Exp Med. 2024 Jul 15;24(1):158. doi: 10.1007/s10238-024-01386-0.
Primary immune thrombocytopenia (ITP) is an acquired autoimmune disorder characterized by an isolated decrease in platelets below 100 × 10/l after the exclusion of other conditions associated with thrombocytopenia. We investigated the role of different memory T-cell subsets, including T stem cell memory (T), in children diagnosed with primary ITP and its association with therapeutic duration. This case-control study included 39 pediatric patients with acute ITP admitted to the Children's Hospital at Assiut University. Using a FACSCanto flow cytometer, CD8 + and CD4 + T-lymphocytes were gated. Five different subsets were characterized in each of these cells according to CD45RO and CD45RA expression. Afterward, gating was performed based on CCR7, CD95, and CD27. Examination of the CD8 + T cells subpopulation showed that Central memory T (T) and CD8 Naïve T (T) cells were significantly lower in ITP patients than in healthy children (p < 0.0001) and (p = 0.01), respectively. In addition, CD8 + T was significantly higher in ITP children than in controls (p = 0.001). CD4 + T cells were significantly lower in the ITP patient group (p = 0.04). However, CD4 + T was significantly higher in patients than controls (p = 0.04). Our research found that ITP patients had an imbalance in the ratio of CD4 to CD8 T cells in the peripheral blood and that T cells may be involved in the pathogenetic mechanism of ITP. T could help in prediction of patients with higher risk of developing ITP.
原发性免疫性血小板减少症（ITP）是一种获得性自身免疫性疾病，其特征是在排除其他与血小板减少相关的疾病后，血小板计数孤立性降至 100×10/l 以下。我们研究了不同记忆 T 细胞亚群，包括 T 干细胞记忆（T），在诊断为原发性 ITP 的儿童中的作用及其与治疗持续时间的关系。这项病例对照研究包括 39 名急性 ITP 患儿，他们均入住 Assiut 大学儿童医院。使用 FACSCanto 流式细胞仪，对 CD8+和 CD4+T 淋巴细胞进行门控。根据 CD45RO 和 CD45RA 的表达，在这些细胞中的每一种细胞中都鉴定出 5 种不同的亚群。之后，根据 CCR7、CD95 和 CD27 进行门控。检查 CD8+T 细胞亚群发现，ITP 患者的中央记忆 T（T）和 CD8 幼稚 T（T）细胞明显低于健康儿童（p<0.0001）和（p=0.01），分别。此外，ITP 患儿的 CD8+T 明显高于对照组（p=0.001）。ITP 患者组的 CD4+T 细胞明显较低（p=0.04）。然而，CD4+T 在患者中明显高于对照组（p=0.04）。我们的研究发现，ITP 患者外周血中 CD4 与 CD8 T 细胞的比例失衡，T 细胞可能参与了 ITP 的发病机制。T 可能有助于预测发生 ITP 的风险较高的患者。
