Stanhope G B, Wolf M K, Billings-Gagliardi S
Brain Res. 1986 Jan;389(1-2):109-16. doi: 10.1016/0165-3806(86)90178-1.
Organotypic cultures of newborn mouse cerebellum, treated with 'antimitotic' drugs to prevent myelination, contain abundant large neurons and axons but no mature oligodendroglia and no myelin. When oligodendroglia, contained in optic nerve fragments from 7-12-day-old normal or shiverer mutant mouse, are added to the cultures, myelin is formed in the vicinity of the optic nerve explant. The phenotype of the added myelin corresponds to the genotype of the optic nerve added, indicating that the added oligodendroglia are making the myelin themselves rather than contributing a diffusible factor which stimulates native cerebellar oligodendroglia or their precursors. This system will be valuable for direct, detailed comparisons of myelin formation by normal and mutant oligodendrocytes.
用“抗有丝分裂”药物处理新生小鼠小脑的器官型培养物以防止髓鞘形成，其中含有丰富的大神经元和轴突，但没有成熟的少突胶质细胞和髓鞘。当将来自7至12日龄正常或颤抖突变小鼠视神经片段中的少突胶质细胞添加到培养物中时，在视神经外植体附近会形成髓鞘。添加的髓鞘的表型与添加的视神经的基因型相对应，这表明添加的少突胶质细胞自身正在形成髓鞘，而不是贡献一种可扩散因子来刺激天然小脑少突胶质细胞或其前体。该系统对于直接、详细比较正常和突变少突胶质细胞的髓鞘形成将是有价值的。
