大鼠培养的少突胶质细胞对小鼠小脑外植体的髓鞘形成

Myelination of mouse cerebellar explants by rat cultured oligodendrocytes.

作者信息

Nishimura R N, Blank N K, Tiekotter K L, Cole R, de Vellis J

出版信息

Brain Res. 1985 Jun 24;337(1):159-62. doi: 10.1016/0006-8993(85)91623-3.

Abstract

It has been shown that transplanted central nervous system tissue containing oligodendrocytes will myelinate neuronal processes in vitro and in situ. In this study we propose to show that cultured rat oligodendrocytes have the capacity to myelinate mouse cerebellar neuronal processes in vitro. Cultured rat oligodendrocytes were transplanted to cytosine arabinoside-treated mouse cerebellar explant cultures, then observed for myelination. Ultrastructural examination showed myelin and myelin-like figures in co-cultures. Control cytosine arabinoside-treated cultures and cultured oligodendroglia were without compact myelin.

摘要

已经表明，含有少突胶质细胞的移植中枢神经系统组织在体外和原位均能使神经元突起髓鞘化。在本研究中，我们旨在表明培养的大鼠少突胶质细胞在体外具有使小鼠小脑神经元突起髓鞘化的能力。将培养的大鼠少突胶质细胞移植到经阿糖胞苷处理的小鼠小脑外植体培养物中，然后观察髓鞘形成情况。超微结构检查显示共培养物中有髓鞘和髓鞘样结构。经阿糖胞苷处理的对照培养物和培养的少突胶质细胞中没有致密髓鞘。