Benziane Anass, Huntošová Veronika, Pevná Viktória, Zauška Luboš, Vámosi György, Hovan Andrej, Zelenková Gabriela, Zeleňák Vladimír, Almáši Miroslav
Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary.
Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Šafárik University in Košice, Jesenná 5, SK-041 54 Košice, Slovak Republic; Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Dúbravská cesta 9, SK-840 05 Bratislava, Slovak Republic.
J Photochem Photobiol B. 2024 Dec;261:113046. doi: 10.1016/j.jphotobiol.2024.113046. Epub 2024 Oct 28.
Transport systems are developed to improve the solubility of the transported drug, increase its stability, enhance its pharmacological activity and target cancer while minimising side effects. In this work, nanoporous silica particles that can be functionalized and loaded with a large number of hydrophobic molecules are proposed. The designed system was modified with folic acid to target the folic acid receptors of cancer cells. This modification enabled a higher uptake of the drug by the cells. Hypericin was selected as a hydrophobic molecule/drug with photodynamic properties suitable for diagnosis and therapy. Fluorescence microscopy and flow cytometry were used to detect the targeting and distribution of hypericin in the cancer cells. Furthermore, the combination of folic acid and hypericin has been shown to form singlet oxygen and to have a synergistic effect in improving the efficacy of photodynamic therapy. The functionalisation of the particles proposed in this work holds great potential for the delivery of hydrophobic drugs to other types of cancer cells with increased expression of the folic acid receptor to which the particles can be attached.
开发运输系统是为了提高被运输药物的溶解度、增加其稳定性、增强其药理活性并靶向癌症，同时将副作用降至最低。在这项工作中，提出了一种可功能化并负载大量疏水分子的纳米多孔二氧化硅颗粒。所设计的系统用叶酸进行了修饰，以靶向癌细胞的叶酸受体。这种修饰使得细胞对药物的摄取更高。金丝桃素被选作具有适合诊断和治疗的光动力特性的疏水分子/药物。使用荧光显微镜和流式细胞术来检测金丝桃素在癌细胞中的靶向性和分布。此外，已证明叶酸和金丝桃素的组合可形成单线态氧，并在提高光动力疗法的疗效方面具有协同作用。这项工作中提出的颗粒功能化对于将疏水性药物递送至叶酸受体表达增加的其他类型癌细胞具有巨大潜力，这些颗粒可以附着于该受体。
