Khidair I A, Teale D M, Potter C W, Rees R C
Cancer. 1986 Apr 15;57(8):1522-7. doi: 10.1002/1097-0142(19860415)57:8<1522::aid-cncr2820570814>3.0.co;2-o.
The plasminogen activator (PA) content of a primary HSV-2-induced hamster fibrosarcoma and sublines derived from its in vivo metastases was investigated using the indirect 125I-labeled fibrin plate method. Fresh tissue culture lines established from primary HSV-2-333-2-26 tumors were shown to produce levels of PA similar to sublines derived from lung or kidney foci that developed after resection of primary tumors. In comparison, normal hamster embryo fibroblast (NHEF) and baby hamster kidney cells produced little or no PA, although baby hamster lung fibroblasts produced intermediate levels of PA. In addition, no correlation was found between PA levels of the sublines and their ability to metastasize from subcutaneous tumors, although tumor cell lines were shown to produce significantly more PA than normal cells.
采用间接¹²⁵I标记纤维蛋白平板法，研究了原发性单纯疱疹病毒2型（HSV - 2）诱导的仓鼠纤维肉瘤及其体内转移衍生亚系中的纤溶酶原激活剂（PA）含量。从原发性HSV - 2 - 333 - 2 - 26肿瘤建立的新鲜组织培养系显示，其产生的PA水平与原发性肿瘤切除后从肺或肾病灶衍生的亚系相似。相比之下，正常仓鼠胚胎成纤维细胞（NHEF）和幼仓鼠肾细胞产生很少或不产生PA，尽管幼仓鼠肺成纤维细胞产生中等水平的PA。此外，虽然肿瘤细胞系产生的PA明显多于正常细胞，但未发现亚系的PA水平与其从皮下肿瘤转移的能力之间存在相关性。
