Coen D, Bottazzi B, Bini A, Conforti M G, Mantovani A, Mussoni L, Donati M B
Int J Cancer. 1983 Jul 15;32(1):67-70. doi: 10.1002/ijc.2910320111.
In order to investigate the possible correlation between plasminogen activator (PA) activity and metastatic potential of tumour cells, we studied cultured cells from the murine fibrosarcoma mFS6 and from its two sublines M4 and M9 which differ markedly in their capacity to cause spontaneous metastases in the lung. PA activity was detected in all the sublines by an amidolytic method and was almost completely inhibited by treatment with antiurokinase antiserum. No significant differences were shown between mFS6, M4 and M9. Moreover, molecular analysis of PA by SDS-PAGE electrophoresis and fibrin overlay revealed in all the cell types a single species having a mol. wt. of approximately 48,000 daltons. Thrombin treatment dramatically inhibited the amidolytic activity of all cells, suggesting a role for this enzyme in the modulation of fibrin formation and dissolution within the primary neoplasm.
为了研究纤溶酶原激活物（PA）活性与肿瘤细胞转移潜能之间的可能相关性，我们研究了来自小鼠纤维肉瘤mFS6及其两个亚系M4和M9的培养细胞，这两个亚系在引起肺部自发转移的能力上有显著差异。通过酰胺分解法在所有亚系中检测到PA活性，并且用抗尿激酶抗血清处理几乎完全抑制了该活性。mFS6、M4和M9之间未显示出显著差异。此外，通过SDS-PAGE电泳和纤维蛋白覆盖对PA进行分子分析，发现在所有细胞类型中均有一种分子量约为48,000道尔顿的单一物质。凝血酶处理显著抑制了所有细胞的酰胺分解活性，表明该酶在原发性肿瘤内纤维蛋白形成和溶解的调节中起作用。
