Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Mol Cell. 2024 Nov 7;84(21):4224-4238.e9. doi: 10.1016/j.molcel.2024.09.031. Epub 2024 Oct 30.
While it is known that temperature sensors trigger calcium (Ca) signaling to confer cold tolerance in cells, less is known about sensors that couple with other secondary messengers. Here, we identify a cold sensor complex of CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) and osmotin-like 1 (OSM1), which triggers 2',3'-cyclic adenosine monophosphate (2',3'-cAMP) production to enhance cold tolerance in rice. COLD6, which is encoded by a major quantitative trait locus (QTL) gene, interacts with the rice G protein α subunit (RGA1) at the plasma membrane under normal conditions. Upon exposure to chilling, cold-induced OSM1 binds to COLD6, kicking out RGA1 from interaction. This triggers an elevation of 2',3'-cAMP levels for enhancing chilling tolerance. Genetic data show that COLD6 negatively regulates cold tolerance and functionally depends on OSM1 in chilling stress. COLD6 alleles were selected during rice domestication. Knockout and natural variation of COLD6 in hybrid rice enhanced chilling tolerance, hinting design potential for breeding. This highlighted a module triggering 2',3'-cAMP to improve chilling tolerance in crops.
虽然已知温度传感器通过触发钙 (Ca) 信号来赋予细胞耐寒性，但对于与其他第二信使偶联的传感器知之甚少。在这里，我们鉴定了一个冷传感器复合物 CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) 和类渗透压蛋白 1 (OSM1)，它触发 2',3'-环腺苷单磷酸 (2',3'-cAMP) 的产生，从而增强水稻的耐寒性。COLD6 由一个主要的数量性状位点 (QTL) 基因编码，在正常条件下与水稻 G 蛋白 α 亚基 (RGA1) 在质膜上相互作用。在受到冷胁迫时，冷诱导的 OSM1 与 COLD6 结合，将 RGA1 从相互作用中逐出。这引发了 2',3'-cAMP 水平的升高，以增强耐寒性。遗传数据表明，COLD6 负调控耐寒性，在冷胁迫下功能上依赖于 OSM1。COLD6 等位基因在水稻驯化过程中被选择。在杂交水稻中敲除和自然变异 COLD6 增强了耐寒性，暗示了在作物中进行品种选育的潜力。这突出了一个模块，通过触发 2',3'-cAMP 来提高作物的耐寒性。
