Khan Muhammad Imran, Zhao Ronghua, Batool Fakhra, Khan Abbas Raza, Ali Rizwan, Hu Yi, Tian Jie, Chen Ni, Qiu Bensheng, Li Fenfen
Medical Imaging Center, Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei 230026, Anhui, China; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, 230027, China; Department of Pathology, District Headquarters Hospital, Jhang 35200, Punjab province, Pakistan.
Medical Imaging Center, Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei 230026, Anhui, China.
Int J Biol Macromol. 2024 Dec;282(Pt 6):137079. doi: 10.1016/j.ijbiomac.2024.137079. Epub 2024 Oct 29.
Anticancer chemotherapies damage normal tissues, and various drug carriers are under consideration to address this issue. Metal-organic frameworks (MOFs) are promising due to their drug-carrying capacity and tunable properties. Target-inspired surface functionalization may further magnify their potential. In this study, we aim to investigate the anticancer activity of reduced iron-based MOFs (RMOFs) functionalized with ferritin and folic acid and loaded with doxorubicin. Successful synthesis and functionalization are confirmed by electron microscopes, Fourier transform infrared spectroscopy and X-ray diffraction. Anticancer activity is evaluated in different tumor cell lines at various doses. Results indicate that the folic acid functionalized and Dox loaded MOF (FADMOF) showed maximum anticancer potential, leaving only 6 % of 4 T1 cells alive at the highest dose. Ferritin and combo MOFs followed closely, while the plain RMOF lagged behind. Furthermore, in HeLa cells, the RMOF showed the best cytotoxicity, reducing cell viability to a mere 30 % at the highest dose. Similarly, in MCF7 cells, theRMOF) showed the best cytotoxicity, reducing cell viability in a dose-dependent manner and to a mere 25 % at the highest dose. Taken together, this study shows that functionalized MOFs have promising anticancer potential across various cancer cell lines. However, loading with doxorubicin reduces their cytotoxicity.
抗癌化疗会损伤正常组织，人们正在考虑使用各种药物载体来解决这一问题。金属有机框架（MOF）因其载药能力和可调节性质而颇具前景。受靶点启发的表面功能化可能会进一步放大其潜力。在本研究中，我们旨在研究用铁蛋白和叶酸功能化并负载阿霉素的还原铁基MOF（RMOF）的抗癌活性。通过电子显微镜、傅里叶变换红外光谱和X射线衍射确认了成功的合成和功能化。在不同剂量下对不同肿瘤细胞系的抗癌活性进行了评估。结果表明，叶酸功能化且负载阿霉素的MOF（FADMOF）显示出最大的抗癌潜力，在最高剂量下仅6%的4T1细胞存活。铁蛋白和复合MOF紧随其后，而普通RMOF则落后。此外，在HeLa细胞中，RMOF表现出最佳的细胞毒性，在最高剂量下将细胞活力降低至仅30%。同样，在MCF7细胞中，RMOF表现出最佳的细胞毒性，并以剂量依赖的方式降低细胞活力，在最高剂量下降至仅25%。综上所述，本研究表明功能化的MOF在各种癌细胞系中具有有前景的抗癌潜力。然而，负载阿霉素会降低它们的细胞毒性。
