State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral and Maxillofacial-Head and Neck Oncology, School of Stomatology-Hospital of Stomatology, Wuhan University, Wuhan, China.
Int J Oral Sci. 2024 Nov 1;16(1):61. doi: 10.1038/s41368-024-00324-w.
The oral and maxillofacial region comprises a variety of organs made up of multiple soft and hard tissue, which are anatomically vulnerable to the pathogenic factors of trauma, inflammation, and cancer. The studies of this intricate entity have been long-termly challenged by a lack of versatile preclinical models. Recently, the advancements in the organoid industry have provided novel strategies to break through this dilemma. Here, we summarize the existing biological and engineering approaches that were employed to generate oral and maxillofacial organoids. Then, we detail the use of modified co-culture methods, such as cell cluster co-inoculation and air-liquid interface culture technology to reconstitute the vascular network and immune microenvironment in assembled organoids. We further retrospect the existing oral and maxillofacial assembled organoids and their potential to recapitulate the homeostasis in parental tissues such as tooth, salivary gland, and mucosa. Finally, we discuss how the next-generation organoids may benefit to regenerative and precision medicine for treatment of oral-maxillofacial illness.
口腔颌面区域由多种器官组成，这些器官由多种软、硬组织构成，在解剖学上容易受到创伤、炎症和癌症等致病因素的影响。由于缺乏多功能的临床前模型，对这一复杂实体的研究一直具有挑战性。最近，类器官产业的进步为突破这一困境提供了新的策略。在这里，我们总结了现有的用于生成口腔颌面类器官的生物学和工程方法。然后，我们详细介绍了使用改良的共培养方法，如细胞簇共接种和气液界面培养技术，在组装类器官中重建血管网络和免疫微环境。我们进一步回顾了现有的口腔颌面组装类器官及其在 recapitulate 亲本组织（如牙齿、唾液腺和粘膜）中的稳态的潜力。最后，我们讨论了下一代类器官如何有益于口腔颌面疾病的再生和精准医学治疗。
