白细胞介素-4 及其受体α在加纳病例对照研究中非复杂性小儿疟疾患者中的表达。
Interleukin-4 and its receptor alpha in paediatric uncomplicated malaria patients from a Ghanaian case-control study.
机构信息
Department of Chemical Pathology, University of Ghana, Korle-Bu, Accra, Ghana.
Department of Paediatric and Adolescent Medicine, Paediatric Haematology and Oncology, University Hospital Münster, 48149, Münster, Germany.
出版信息
BMC Res Notes. 2024 Oct 31;17(1):328. doi: 10.1186/s13104-024-06984-5.
OBJECTIVES
This study investigated gene polymorphisms in the interleukin-4 (IL-4) and its receptor alpha (IL-4Rα) gene regions in human hosts with uncomplicated malaria.
DATA DESCRIPTION
Blood samples were obtained from a case-control study conducted at the Sogakope district hospital in the Volta region of Ghana. Thick blood films were made and used to detect the presence and levels of parasitaemia in the patient samples. Genotyping of IL-4 (150 C/T) and the IL-4Rα (Pro-478-Ser) polymorphisms in the promoter regions and receptor gene was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) after DNA extraction. The data are useful in determining genetic polymorphisms (allele and genotypic frequencies) of IL-4 and its alpha receptor. In addition, they are useful when comparing levels of parasite density and haematological parameters between genotypic variants of IL-4 and IL-4Rα. These data contribute to our understanding of the genetic basis of malaria susceptibility, particularly in the population of the Volta region of Ghana.
目的
本研究调查了人宿主中非复杂性疟疾患者白细胞介素-4（IL-4）及其受体α（IL-4Rα）基因区域的基因多态性。
数据描述
从加纳沃尔特地区索科卡佩区医院进行的病例对照研究中采集了血液样本。制作厚血涂片并用于检测患者样本中寄生虫血症的存在和水平。在提取 DNA 后，使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对白细胞介素-4（150 C/T）和白细胞介素-4Rα（Pro-478-Ser）多态性在启动子区域和受体基因中的基因分型。这些数据可用于确定白细胞介素-4 和其α受体的遗传多态性（等位基因和基因型频率）。此外，当比较白细胞介素-4 和白细胞介素-4Rα的基因型变异体之间的寄生虫密度和血液学参数水平时，这些数据也很有用。这些数据有助于我们理解疟疾易感性的遗传基础，特别是在加纳沃尔特地区的人群中。
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