Pugliano Maria Giulia, Cosenza Gianfranco, D'Anza Emanuele, Fulgione Andrea, Murru Nicoletta, Paolo Marika Di, Albarella Sara, Peretti Vincenzo, Ciotola Francesca
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Department of Agriculture, University of Napoli Federico II, Portici, Italy.
Anim Biosci. 2025 May;38(5):863-872. doi: 10.5713/ab.24.0323. Epub 2024 Oct 25.
The Bagnolese sheep is an authochtonous dual-purpose breed (milk and meat) reared in the Campania region, whose milk is used to produce Pecorino Bagnolese cheese. Genetic information on this sheep is extremely limited, especially regarding genes affecting productions. The aim of this study was to investigate milk production traits in Bagnolese sheep and the variability of diacylglycerol acyltransferase 1 (DGAT1) gene and its effects on milk production.
Milk quantity was recorded during the morning milking, while Kjeldahl and Gerber methods were used to assess protein and fat percentage (w/v) of collected milk samples. Two polymerase chain reaction-restriction fragment length polymorphism protocols using BamHI and MspI endonucleases for genotyping of g.5553C>T and g.8539C>T at DGAT1 locus, respectively, were set up.
Bagnolese sheep milk shows high fat and protein concentrations. Genotyping revealed a high frequency of the g.5553C and g.8539C alleles (0.56 and 0.95, respectively). The association study between the single nucleotide polymorphism (SNP) g.5553C>T and milk traits showed that animals with the CT genotype had a higher percentage of fat produced per milking than those with the CC and TT genotypes (p<0.01). Similar results were found for protein yield percentage, with CT individuals being more productive than CC individuals (p<0.01).
Bagnolese sheep milk parameters found are associated with high yields in the resulting dairy products. CT genotype at the SNP g.5553 of DGAT1 has shown a positive association with fat and protein milk yield percentage suggesting it could be considered a marker to improve productions of this breed. Finally, the new genotyping techniques used for this study enable a cheap and reliable characterization of two DGAT1 SNPs in sheep.
巴尼奥莱塞羊是一种原产于坎帕尼亚地区的兼用型品种（乳用和肉用），其羊奶用于生产巴尼奥莱塞佩科里诺奶酪。关于这种羊的遗传信息极为有限，尤其是涉及影响生产性能的基因方面。本研究的目的是调查巴尼奥莱塞羊的产奶性状、二酰甘油酰基转移酶1（DGAT1）基因的变异性及其对产奶量的影响。
在早晨挤奶时记录产奶量，同时采用凯氏定氮法和盖勃法评估所采集奶样的蛋白质和脂肪百分比（w/v）。分别建立了两种聚合酶链反应-限制性片段长度多态性分析方法，使用BamHI和MspI内切酶对DGAT1基因座上的g.5553C>T和g.8539C>T进行基因分型。
巴尼奥莱塞羊的羊奶显示出高脂肪和高蛋白质含量。基因分型显示g.5553C和g.8539C等位基因的频率较高（分别为0.56和0.95）。单核苷酸多态性（SNP）g.5553C>T与产奶性状的关联研究表明，CT基因型的动物每次挤奶产生的脂肪百分比高于CC和TT基因型的动物（p<0.01）。蛋白质产量百分比也有类似结果，CT个体比CC个体的生产性能更高（p<0.01）。
所发现巴尼奥莱塞羊的羊奶参数与所生产乳制品的高产量相关。DGAT1基因g.5553位点的CT基因型与羊奶脂肪和蛋白质产量百分比呈正相关，表明它可被视为提高该品种生产性能的一个标记。最后，本研究中使用的新基因分型技术能够对绵羊的两个DGAT1 SNP进行廉价且可靠的特征分析。
