Peguero Ricardo L, Bell Nicole A, Bimbo-Szuhai Andras, Roach Kevin D, Fulop Zoltan L, Corbo Christopher P
Laboratory of Developmental Brain Research & Neuroplasticity, Department of Biological Sciences, Wagner College, Staten Island, NY 10301, USA;
NeuroSci. 2022 Apr 2;3(2):186-199. doi: 10.3390/neurosci3020014. eCollection 2022 Jun.
It has been shown that adult zebrafish are capable of regenerating regions of the central nervous system (CNS) after insult. Unlike in higher-order vertebrates where damage to the CNS leads to glial scar formation and permanent functional deficits, damage to the adult zebrafish CNS is transient and followed by nearly complete reconstitution of both function and anatomy. Our lab's previous work has shown that explants of zebrafish optic tectum can survive in organotypic culture for up to 7 days, and that at 96 h in culture, regenerating cells of the tectum begin to form structures that resemble the embryonic neural tube seen in vertebrate development. The current project aims to elucidate the cellular and ultrastructural components of the formation of this neural tube-like structure using scanning and transmission electron microscopy. Our results show that after injury and cultivation for 96 h, the explants contained differentiating cells that were undergoing several cellular events, such as neovascularization, and rosette/cisternae formation, leading to the formation of a structure resembling the embryonic neural tube. Additionally, we demonstrate healthy cellular ultrastructures in both degenerated and regenerated areas of the explant.
研究表明，成年斑马鱼在受到损伤后能够再生中枢神经系统（CNS）的区域。与高等脊椎动物不同，在高等脊椎动物中，中枢神经系统受损会导致胶质瘢痕形成和永久性功能缺陷，而成年斑马鱼中枢神经系统的损伤是短暂的，随后功能和解剖结构几乎完全重建。我们实验室之前的工作表明，斑马鱼视顶盖外植体在器官型培养中可以存活长达7天，并且在培养96小时时，视顶盖的再生细胞开始形成类似于脊椎动物发育中所见胚胎神经管的结构。当前项目旨在使用扫描电子显微镜和透射电子显微镜阐明这种神经管样结构形成的细胞和超微结构成分。我们的结果表明，在损伤并培养96小时后，外植体中含有正在经历多种细胞事件的分化细胞，如新生血管形成以及玫瑰花结/池状结构形成，从而导致形成类似于胚胎神经管的结构。此外，我们在外植体的退化和再生区域均展示了健康的细胞超微结构。
