一种用于血清脂肪酶检测校准的酶法。

An enzymatic method for calibration of serum lipase assays.

作者信息

Hoffmann G E, Neumann U, Hoffmann S, Kaspar P, Weiss L

出版信息

Clin Chem. 1986 Mar;32(3):545-7.

Abstract

An enzymatic method for the calibration of a turbidimetric lipase assay is described, based on measurement of free fatty acids liberated by the action of lipase. The substrate of the turbidimetric assay is a colipase-containing triolein emulsion. For determination of the free fatty acids a commercial test kit including acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase is used. Intra- and interassay imprecision (CV) is about 5% at above-normal lipase activities, about 10% at normal values. Temperature coefficients are 1.24 and 1.45, respectively, for measurements at 30 and 37 degrees C vs 25 degrees C.

摘要

本文描述了一种基于测量脂肪酶作用释放的游离脂肪酸来校准比浊法脂肪酶测定的酶法。比浊法测定的底物是含辅脂肪酶的三油酸甘油酯乳液。测定游离脂肪酸时使用了一种包含酰基辅酶A合成酶、酰基辅酶A氧化酶和过氧化物酶的商业检测试剂盒。在脂肪酶活性高于正常水平时，批内和批间不精密度（CV）约为5%，在正常数值时约为10%。与25℃相比，在30℃和37℃测量时的温度系数分别为1.24和1.45。