School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, P. R. China.
SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Dalian, Liaoning 116034, P. R. China.
J Agric Food Chem. 2024 Nov 13;72(45):25436-25448. doi: 10.1021/acs.jafc.4c08203. Epub 2024 Nov 1.
Postbiotics have emerged as a promising alternative to probiotics. However, it remains unclear whether postbiotics can exert regulatory effects on intestinal flora and metabolism as probiotics. Thus, we investigated the effects of probiotic and postbiotic in rats with whey protein-induced food allergy, which demonstrated that postbiotic intervention effectively alleviated allergy symptoms, reduced serum immunoglobulin E (IgE) and mast cell protease-1 (mMCP-1) levels, and regulated the type helper 1 cell/2 cell (Th1/Th2) balance in both serum and spleen. Metagenomic analysis revealed that postbiotics induced more significant changes in intestinal flora. Untargeted metabolomics analysis showed that both probiotics and postbiotics significantly up-regulated various differential metabolites, which were negatively correlated with immune indices, including malvidin-3-glucoside, 3,4-dihydroxymandelic acid, nicotinamide, triterpenoids, pirbuterol, and 4-hydroxybenzoic acid. This study confirms that postbiotics can alleviate food allergies and regulate intestinal flora and metabolites, which provides a valuable reference for the use of postbiotics in mitigating allergic diseases through gut microbiota and metabolite modulation.
后生元作为益生菌的替代品而备受关注。然而，后生元是否可以像益生菌一样对肠道菌群和代谢产生调节作用尚不清楚。因此，我们研究了后生元和益生菌对乳清蛋白诱导的食物过敏大鼠的影响，结果表明后生元干预能有效缓解过敏症状，降低血清免疫球蛋白 E（IgE）和肥大细胞蛋白酶-1（mMCP-1）水平，并调节血清和脾脏中辅助性 1 型/2 型细胞（Th1/Th2）平衡。宏基因组分析显示后生元诱导了肠道菌群更显著的变化。非靶向代谢组学分析表明，益生菌和后生元都能显著上调多种差异代谢物，这些代谢物与免疫指标呈负相关，包括矢车菊素-3-葡萄糖苷、3,4-二羟基苯乙酸、烟酰胺、三萜类化合物、吡布特罗和对羟基苯甲酸。本研究证实后生元可以缓解食物过敏，调节肠道菌群和代谢物，为通过肠道微生物群和代谢物调节后生元缓解过敏疾病提供了有价值的参考。
