Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 980-0845, Japan.
Arch Virol. 2024 Nov 1;169(11):235. doi: 10.1007/s00705-024-06162-9.
Two Ralstonia phages, FLC1-1B and FLC4-3B, were isolated from leaf litter compost, using Ralstonia pseudosolanacearum, which is a causal agent of bacterial wilt disease, as a host. The genomic DNA sequences of FLC1-1B and FLC4-3B were determined and found to be 290,008 bp and 291,257 bp in length, respectively, and they were therefore classified as jumbo phages. However, they did not show high similarity to any jumbo phage genomic sequence in the NCBI nt database. The closest hit in a BLAST search was the jumbo phage ripduovirus RP12, with only 35% coverage and 77% sequence identity, whereas the FLC1-1B and FLC4-3B sequences were 99.0% identical. Based on these findings, FLC1-1B and FLC4-3B should be classified as members of a new genus in the order Caudoviricetes. FLC4-3B was found to suppress wilt disease in tomato plants, suggesting that it has potential as a biocontrol agent for managing R. pseudosolanacearum infections.
两种罗尔斯顿氏菌噬菌体 FLC1-1B 和 FLC4-3B 是从叶屑堆肥中分离出来的，使用罗尔斯顿氏假单胞菌作为宿主，罗尔斯顿氏假单胞菌是细菌性萎蔫病的病原体。FLC1-1B 和 FLC4-3B 的基因组 DNA 序列已被确定，长度分别为 290008bp 和 291257bp，因此被归类为巨型噬菌体。然而，它们与 NCBI nt 数据库中的任何巨型噬菌体基因组序列都没有显示出很高的相似性。BLAST 搜索中最接近的是巨型噬菌体 ripduovirus RP12，只有 35%的覆盖率和 77%的序列同一性，而 FLC1-1B 和 FLC4-3B 序列则有 99.0%的同一性。基于这些发现，FLC1-1B 和 FLC4-3B 应该被归类为长尾病毒目（order Caudoviricetes）中的一个新属的成员。FLC4-3B 被发现能抑制番茄植株的萎蔫病，表明它有作为防治罗尔斯顿氏假单胞菌感染的生物防治剂的潜力。
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