Alwael H, Alzahrani K A, Abduljabbar T N, Alharthi A S, Alshareef F M, Bahaidarah E A, Alkhraije A A, El-Shahawi M S
Department of Chemistry, Faculty of Science, King Abdulaziz University, P. O. Box 80203, Jeddah, 21589, Saudi Arabia.
Center of Excellence for Advanced Materials Research (CEAMR), King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
J Fluoresc. 2024 Nov 1. doi: 10.1007/s10895-024-03999-w.
The current study reports a novel fluorescent chemosensor based on the tagging agent 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein (Rose Bengal, RB) for detection of trace levels of Hgin environmental water. The established probe has been based upon liquid-liquid extraction (LLE) of the developed ternary complex ion associate {[Hg (bpy)].[RB]} of Hg -2,2- bipyridyl complex [Hg (bpy)] and RB at pH 9.0 onto chloroform and measuring the resulting fluorescence enhancement signal intensity at λ = 570/ (580-600) nm. The limits of detection (LOD) and quantification (LOQ) of for Hg was calculated to be 6.06 and 20 nM with a linear dynamic range (LDR) of 0.02-20µM, respectively. The stability constant, stoichiometry, chemical equilibria, and the thermodynamic parameters (ΔH, ΔS, and ΔG) of the developed ion associate were evaluated and assigned. Student's t and F tests at 95% confidence were fruitfully used for validation of the proposed methodology for Hg detection in water samples with the aid of inductively coupled plasma-optical emission spectrometry (ICP-OES). The established strategy was successfully applied for detection of trace levels of Hg in water samples with acceptable results. The proposed probe was satisfactorily applied for total determination and speciation of Hg in various water samples. Integrating the functional chelating agent onto associate formation to improve the selectivity and sensing properties of the LLE combining sensing probe towards target analyte in water represent the main interest.
本研究报道了一种基于标记剂4,5,6,7-四氯-2',4',5',7'-四碘荧光素（孟加拉玫瑰红，RB）的新型荧光化学传感器，用于检测环境水中痕量的汞。所建立的探针基于在pH 9.0条件下，将汞-2,2-联吡啶配合物[Hg(bpy)]与RB形成的三元络合离子缔合物{[Hg(bpy)].[RB]}通过液-液萃取（LLE）到氯仿中，并测量在λ = 570/（580 - 600）nm处产生的荧光增强信号强度。计算得出汞的检测限（LOD）和定量限（LOQ）分别为6.06 nM和20 nM，线性动态范围（LDR）为0.02 - 20 μM。评估并确定了所形成的离子缔合物的稳定常数、化学计量比、化学平衡以及热力学参数（ΔH、ΔS和ΔG）。在95%置信度下的学生t检验和F检验被有效地用于借助电感耦合等离子体发射光谱法（ICP - OES）验证所提出的水中汞检测方法。所建立的策略成功应用于水样中痕量汞的检测，结果令人满意。所提出的探针令人满意地应用于各种水样中汞的总量测定和形态分析。将功能性螯合剂整合到缔合物形成过程中，以提高LLE结合传感探针对水中目标分析物的选择性和传感性能是主要研究方向。
