Liu Xuanyu, Hu Junjie, Hu Yinchun, Liu Yeying, Wei Yan, Huang Di
Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Colloids Surf B Biointerfaces. 2025 Jan;245:114346. doi: 10.1016/j.colsurfb.2024.114346. Epub 2024 Oct 29.
Uncontrolled bleeding from incompressible or irregularly shaped wounds is a major factor in the death of people in the battlefield or surgery process. Ideal rapid hemostatic materials should have the performance of rapid hemostasis and at the same time can be applied to a variety of complex wound trauma types, in addition, excellent antimicrobial properties, adhesion, biocompatibility, degradation, and the non-toxicity of degradation products are also necessary, but there are fewer hemostatic materials that meet these requirements. Herein, we prepared an injectable hemostatic hydrogel based on the natural products sodium alginate (SA) and carboxymethyl chitosan (CMC). Oxidized sodium alginate (OSA) was prepared by the oxidation reaction of NaIO with SA, and OSA with aldehyde group was mixed with CMC with amino group to rapidly form an in situ injectable hemostatic hydrogel (OSA/CMC) by the Schiff base reaction. OSA/CMC hydrogel exhibited excellent antimicrobial and adhesion properties by the Schiff base reaction. In addition, OSA/CMC hydrogel directly activate the endogenous coagulation pathway through the synergistic effect of OSA, CMC to enhance the hemostatic effect. The results of in vivo hemostasis study showed that OSA/CMC hydrogel significantly accelerated hemostasis and reduced blood loss in liver hemorrhage model and tail amputation model. Therefore, OSA/CMC hydrogel is expected to be a potential material in the direction of rapid clinical hemostasis due to its good adhesion properties, antimicrobial properties, biocompatibility, blood compatibility, and efficient rapid hemostasis.
来自不可压缩或形状不规则伤口的失控出血是战场上或手术过程中人员死亡的主要因素。理想的快速止血材料应具有快速止血性能，同时可应用于多种复杂伤口创伤类型，此外，优异的抗菌性能、粘附性、生物相容性、降解性以及降解产物的无毒性也是必需的，但满足这些要求的止血材料较少。在此，我们基于天然产物海藻酸钠（SA）和羧甲基壳聚糖（CMC）制备了一种可注射止血水凝胶。通过NaIO与SA的氧化反应制备氧化海藻酸钠（OSA），将具有醛基的OSA与具有氨基的CMC混合，通过席夫碱反应快速形成原位可注射止血水凝胶（OSA/CMC）。OSA/CMC水凝胶通过席夫碱反应表现出优异的抗菌和粘附性能。此外，OSA/CMC水凝胶通过OSA、CMC的协同作用直接激活内源性凝血途径以增强止血效果。体内止血研究结果表明，OSA/CMC水凝胶在肝出血模型和尾部截肢模型中显著加速止血并减少失血量。因此，OSA/CMC水凝胶因其良好的粘附性能、抗菌性能、生物相容性、血液相容性和高效快速止血性能，有望成为临床快速止血方向的潜在材料。
