Zhai Shaobo, You Jiaqian, Yang Zheng, Liu Yang, He Tianming, Wu Yuchuan, Wei Xuyan, Li Mucong, Shi Xiaolu, Gao Fengxiang, Chu Shunli
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, 130021, Jilin, China.
Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, No.56, Lingyuan West Road, Yuexiu District, Guangzhou, 510055, China.
Mater Today Bio. 2025 May 5;32:101817. doi: 10.1016/j.mtbio.2025.101817. eCollection 2025 Jun.
Wound infection is a serious clinical challenge, often accompanied by persistent inflammatory response and the threat of increased bacterial resistance, there is an urgent requirement to develop a wound dressing with potent antimicrobial as well as immunomodulatory effects. In this study, we developed a multifunctional wound dressing to promote healing of infected wounds by doping puerarin-loaded mesoporous polydopamine nanoparticles into a carboxymethyl chitosan/sodium alginate oxide-based hydrogel. The hydrogel has good injectability, adhesion, and self-healing capabilities, and can be well matched to irregular wounds and against external injuries. The loaded PUE is rapidly released from the hydrogel in response to near-infrared (NIR) laser irradiation, which has a controlled release property. In addition, under NIR laser irradiation, the hydrogel with good photothermal properties exerted good antimicrobial properties through localized high temperature, and exhibited good antioxidant, immune microenvironment modulation, and pro-angiogenic effects through accelerated released PUE. In the E. coli-infected full-thickness skin wounds healing experiments, the hydrogel can effectively kill bacteria, regulate the immune microenvironment, promote collagen deposition and angiogenesis, and significantly accelerate the wound healing, showing promising applications.
伤口感染是一项严峻的临床挑战，常伴有持续的炎症反应以及细菌耐药性增加的威胁，因此迫切需要开发一种具有强大抗菌和免疫调节作用的伤口敷料。在本研究中，我们通过将负载葛根素的介孔聚多巴胺纳米颗粒掺杂到羧甲基壳聚糖/氧化海藻酸钠基水凝胶中，开发了一种多功能伤口敷料，以促进感染伤口的愈合。该水凝胶具有良好的可注射性、粘附性和自愈能力，能够很好地贴合不规则伤口并抵御外部损伤。负载的葛根素（PUE）在近红外（NIR）激光照射下从水凝胶中快速释放，具有控释特性。此外，在近红外激光照射下，具有良好光热性能的水凝胶通过局部高温发挥良好的抗菌性能，并通过加速释放的PUE表现出良好的抗氧化、免疫微环境调节和促血管生成作用。在大肠杆菌感染的全层皮肤伤口愈合实验中，该水凝胶能够有效杀灭细菌、调节免疫微环境、促进胶原蛋白沉积和血管生成，并显著加速伤口愈合，显示出广阔的应用前景。
