Tong Xishuai, Wang Guoshuai, Zhao Xinrui, Zhou Jiatao, Wang Panting, Xia Han, Bian Jianchun, Liu Xuezhong, Yuan Yan, Zou Hui, Liu Zongping, Gu Jianhong
Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, PR China; Jiangsu Key Laboratory of Zoonosis, Yangzhou 225009, Jiangsu, PR China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, PR China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, PR China; Jiangsu Key Laboratory of Zoonosis, Yangzhou 225009, Jiangsu, PR China.
Int J Biol Macromol. 2024 Dec;282(Pt 5):137106. doi: 10.1016/j.ijbiomac.2024.137106. Epub 2024 Oct 30.
Cadmium (Cd), a toxic heavy metal pollutant, inflicts widespread damage on various organs and tissues, including cartilage, where it induces chondrocyte apoptosis. Angelica sinensis polysaccharides (ASP), a key active component of the traditional Chinese medicine Angelica sinensis, have been shown to possess anti-apoptotic effects on chondrocytes. This study investigates the in vitro effects of ASP on alleviating Cd-induced apoptosis in layer chicken chondrocytes, focusing on the mitochondrial apoptosis pathway mediated by the c-Jun N-terminal kinase (JNK) signaling pathway. Chondrocytes were isolated from layer chicken embryos and confirmed to express collagen type II alpha 1 (Col2a1). We found that Cd triggered apoptosis in the chondrocytes; however, the use of the JNK inhibitor SP 600125 mitigated mitochondrial structural damage casused by Cd, indicating the involvement of JNK signaling in this process. Furthermore, ASP effectively alleviated Cd-induced apoptosis in layer chicken chondrocytes by inhibiting JNK signaling in vitro. Our findings provide a theoretical foundation for the clinical application of ASP in preventing Cd-induced cartilage diseases in poultry.
镉（Cd）是一种有毒的重金属污染物，会对包括软骨在内的各种器官和组织造成广泛损害，它会诱导软骨细胞凋亡。当归多糖（ASP）是中药当归的关键活性成分，已被证明对软骨细胞具有抗凋亡作用。本研究调查了ASP在体外对减轻蛋鸡软骨细胞中镉诱导的凋亡的影响，重点关注由c-Jun氨基末端激酶（JNK）信号通路介导的线粒体凋亡途径。从蛋鸡胚胎中分离软骨细胞，并证实其表达II型胶原α1（Col2a1）。我们发现镉会触发软骨细胞凋亡；然而，使用JNK抑制剂SP 600125可减轻镉引起的线粒体结构损伤，表明JNK信号通路参与了这一过程。此外，ASP在体外通过抑制JNK信号通路有效减轻了蛋鸡软骨细胞中镉诱导的凋亡。我们的研究结果为ASP在预防家禽镉诱导的软骨疾病中的临床应用提供了理论基础。
